Thermo Fisher Scientific H3K4me2 Polyclonal Antibody
H3K4me2 변형 히스톤 H3에 특이적인 Rabbit Polyclonal Antibody로, WB, IHC, ChIP, Dot blot 등에 사용 가능. 다양한 종(C. elegans, Human 등)에 반응. 액상 형태, 1.1 mg/mL 농도, -20°C 보관. 연구용으로만 사용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500 |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1:50 |
| ChIP assay (ChIP) | 2–5 µg/1×10⁶ cells |
| Dot blot (DB) | 1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | C. elegans, Chicken, Fruit fly, Human, Mouse, Plant, Rat, Xenopus |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Histone H3 [Dimethyl Lys4] affinity purified antibody prepared from whole rabbit serum immunized with a synthetic dimethylated peptide surrounding Lysine 4 of human Histone H3.2 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 30% glycerol, 0.15M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Store vial at -20°C prior to opening.
- Aliquot contents and freeze at -20°C or below for extended storage.
- Avoid cycles of freezing and thawing.
- Centrifuge product if not completely clear after standing at room temperature.
- Stable for several weeks at 4°C as undiluted liquid.
- Dilute only prior to immediate use.
This antibody reacts with human Histone H3.2. BLAST analysis suggests cross-reactivity with Human, Mouse, and C. elegans. Predicted to react with Rat, Chicken, Xenopus, Drosophila, and Plant based on 100% sequence homology. Cross-reactivity with Histone H3 from other sources has not been determined.
Target Information
Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3, along with four core histone proteins, binds to DNA forming the nucleosome structure. Histones play key roles in transcription regulation, DNA repair, replication, and chromosomal stability. Post-translational modifications such as methylation, acetylation, and phosphorylation of histone tails influence chromatin structure and transcription factor binding.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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