Thermo Fisher Scientific H3K9me1T6ph Polyclonal Antibody
다양한 종에서 반응하는 Rabbit Polyclonal 항체로 H3K9me1T6ph 표적 인식. WB, IHC, ICC, ChIP 등 다양한 응용 가능. 고순도(affinity chromatography) 정제 및 액상 형태로 제공. -20°C 보관으로 안정성 유지.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (IHC) | 1:200 |
| Immunocytochemistry (ICC/IF) | 1:200 |
| ChIP assay (ChIP) | 2–5 µg/1×10⁶ cells |
| Dot blot (DB) | 1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | C. elegans, Chicken, Fruit fly, Human, Mouse, Plant, Rat, Xenopus |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Histone H3 [p Thr6, Monomethyl Lys9]. Affinity purified antibody prepared from whole rabbit serum produced by repeated immunizations with a synthetic monomethylated/phosphorylated peptide surrounding Lysine 9/Threonine 6 of human Histone H3.2. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.85 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 30% glycerol, 0.15M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Store vial at -20°C prior to opening.
- Aliquot contents and freeze at -20°C or below for extended storage.
- Avoid cycles of freezing and thawing.
- Centrifuge product if not completely clear after standing at room temperature.
- Stable for several weeks at 4°C as an undiluted liquid.
- Dilute only prior to immediate use.
This antibody reacts with human Histone H3.2.
A BLAST analysis suggests cross-reactivity with Human, Mouse, and C. elegans.
Predicted to react with Rat, Chicken, Xenopus, Drosophila, and Plant species based on 100% sequence homology.
Cross-reactivity with Histone H3 from other sources has not been determined.
Target Information
Histones are basic nuclear proteins responsible for the nucleosome structure of the chromosomal fiber in eukaryotes.
Approximately 146 bp of DNA wraps around a nucleosome composed of pairs of core histones (H2A, H2B, H3, H4).
Chromatin fiber is compacted through interaction of linker histone H1 with DNA between nucleosomes to form higher-order structures.
This gene, found in a histone cluster on chromosome 1, encodes a member of the histone H3 family.
Transcripts lack polyA tails and contain a palindromic termination element.
This record represents the telomeric copy among four duplicated histone genes in the cluster.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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