Thermo Fisher Scientific H3K27me1 Polyclonal Antibody
H3K27me1 단일메틸화 히스톤 H3 영역에 특이적으로 결합하는 Rabbit Polyclonal 항체. Western blot, ICC/IF, ELISA, ChIP 등 다양한 응용 가능. 고순도 친화 크로마토그래피 정제. -20°C에서 보관, 연구용 전용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:1,000 |
| ELISA | 1:500 |
| ChIP assay (ChIP) | Assay-dependent |
| Peptide Array (Array) | 1:2,000 |
| Dot blot (DB) | 1:20,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Plant |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Raised against the region of histone H3 containing monomethylated lysine 27 (H3K27me1), using a KLH-conjugated synthetic peptide. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.93 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS |
| Contains | 0.05% sodium azide, 0.05% ProClin 300 |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Dry ice |
| RRID | AB_2533863 |
Target Information
Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3, along with four core histone proteins, binds to DNA forming the nucleosome structure. Histones play a central role in transcription regulation, DNA repair, DNA replication, and chromosomal stability.
Post-translational modifications of histones can alter chromatin structure or facilitate binding of specific transcription factors. The N-terminal tail of histone H3 can undergo methylation, acetylation, or phosphorylation, influencing key cellular processes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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