Thermo Fisher Scientific H3K9me2 Polyclonal Antibody
H3K9me2 다메틸화 히스톤 H3 영역을 인식하는 Rabbit Polyclonal 항체로, WB, ICC/IF, ELISA 등 다양한 응용에 적합. 인간, 마우스, 식물, 제노푸스 반응성. 고순도 친화 크로마토그래피 정제. -20°C 보관, 연구용 전용.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000 | - |
| Immunocytochemistry (ICC/IF) | 1:500 | - |
| ELISA | 1:1,000 | - |
| ChIP assay (ChIP) | - | 1 publication |
| Peptide Array (Array) | 0.25 µg/mL | - |
| Dot blot (DB) | 1:20,000 | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Plant, Xenopus |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Raised against the region of histone H3 containing dimethylated lysine 9 (K9me2), using a KLH-conjugated synthetic peptide |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.15 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS |
| Contains | 0.05% sodium azide, 0.05% ProClin 300 |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Dry ice |
| RRID | AB_2533858 |
Target Information
Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3, along with four core histone proteins, binds to DNA forming the structure of the nucleosome.
Histones play a central role in transcription regulation, DNA repair, DNA replication, and chromosomal stability.
Post-translationally, histones are modified in various ways to either directly change chromatin structure or enable binding of specific transcription factors.
The N-terminal tail of histone H3 protrudes from the nucleosome core and can undergo several types of post-translational modification that influence cellular processes, including methylation, acetylation, and phosphorylation of lysine, arginine, serine, or threonine residues.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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