Thermo Fisher Scientific CCL21 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1 µg/mL

-

Immunohistochemistry (Frozen) (IHC (F))

5-15 µg/mL

-

Flow Cytometry (Flow)

2.5 µg per million cells

-

Neutralization (Neu)

0.5-2.5 µg/mL

-

Product Specifications

Species Reactivity

Mouse

Host/Isotype

Goat / IgG

Class

Polyclonal

Type

Antibody

Immunogen

E. coli-derived recombinant mouse CCL21/6Ckine Ser24-Gly133 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:CCL21,uniProtId:P86793-1,ncbiNodeId:10090,antigenRange:24-133,antigenLength:133,antigenImageFileName:PA5-47016_CCL21_P86793-1_Goat.svg,antigenImageFileNamePDP:PA5-47016_CCL21_P86793-1_Goat_PDP.jpeg,sortOrder:2},{targetFamily:CCL21,uniProtId:P84444-1,ncbiNodeId:10090,antigenRange:24-133,antigenLength:133,antigenImageFileName:PA5-47016_CCL21_P84444-1_Goat.svg,antigenImageFileNamePDP:PA5-47016_CCL21_P84444-1_Goat_PDP.jpeg,sortOrder:3},{targetFamily:CCL21,uniProtId:P86792-1,ncbiNodeId:10090,antigenRange:24-133,antigenLength:133,antigenImageFileName:PA5-47016_CCL21_P86792-1_Goat.svg,antigenImageFileNamePDP:PA5-47016_CCL21_P86792-1_Goat_PDP.jpeg,sortOrder:4}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.2 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 5% trehalose

Contains

No Preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2608232

Product Specific Information

In direct ELISAs, approximately 100% cross reactivity with recombinant rat CCL21/6Ckine is observed and less than 15% cross-reactivity with recombinant human CCL21/6Ckine is observed.

Reconstitute at 0.2 mg/mL in sterile PBS.

Endoxin level is <0.10 EU per 1 µg of the antibody by the LAL method.

Target Information

This gene is one of several CC cytokine genes clustered on the p-arm of chromosome 9. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. Similar to other chemokines the protein encoded by this gene inhibits hemopoiesis and stimulates chemotaxis. This protein is chemotactic in vitro for thymocytes and activated T cells, but not for B cells, macrophages, or neutrophils. The cytokine encoded by this gene may also play a role in mediating homing of lymphocytes to secondary lymphoid organs. It is a high affinity functional ligand for chemokine receptor 7 (CCR7) that is expressed on T and B lymphocytes and a known receptor for another member of the cytokine family (small inducible cytokine A19).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.