Thermo Fisher Scientific EBI3 Monoclonal Antibody (V1.4C4.22), Biotin

Applications and Tested Dilutions

Product Specifications

Additional Formats

• Unconjugated
• Request custom conjugation

Product Specific Information

• Store vial at 4°C prior to restoration.
• Restore with 0.1 mL of deionized water (or equivalent).
• For extended storage, aliquot contents and freeze at -20°C or below.
• Avoid cycles of freezing and thawing.
• Centrifuge product if not completely clear after standing at room temperature.
• This product is stable for several weeks at 4°C as an undiluted liquid.
• Dilute only prior to immediate use.

This antibody is specific for mouse EBI3 protein. A BLAST analysis suggests cross-reactivity with EBI3 from mouse sources based on 100% homology with the immunizing sequence. Cross-reactivity with EBI3 from other species has not been determined.

Target Information

EBI3 (Epstein-Barr virus Induced-3) is a secreted glycoprotein of the hematopoietin receptor family. It plays a regulatory role in Th2-type immune responses and Th2-mediated tissue inflammation, possibly through control of iNKT cell function. EBI3 dimerizes with p28 and p35 subunits of IL-12 to form IL-27 and IL-35, respectively.
IL-27 is an early product of activated antigen-presenting cells and negatively regulates Th17 cell differentiation. EBI3 expression in dendritic cells is transcriptionally regulated by TLR signaling via MyD88 and NF-κB during innate immune responses.
EBI3 signaling inhibits delayed-type hypersensitivity responses by suppressing IL-17 production and inducing IL-10 hyperproduction. It may also play a role in controlling tumor metastasis via lung CD8+ T cells. EBI3 deficiency is associated with reduced Th2 cytokine production, affecting allergic airway inflammation in asthma. Targeted deletion of EBI3 protects mice from lung metastasis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.