Thermo Fisher Scientific CD27 Monoclonal Antibody (O323), Brilliant Ultra Violet 737, eBioscience

Applications

• Flow Cytometry (Flow)
  Tested Dilution: 5 µL (0.25 µg)/test

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 395
• Brilliant UV 563
• Brilliant UV 615
• Brilliant UV 805
• Brilliant Violet 421
• Brilliant Violet 480
• Brilliant Violet 605
• Brilliant Violet 711
• eFluor 450
• FITC
• NovaFluor Blue 610-70S
• NovaFluor Blue 660-120S
• NovaFluor Blue 690
• NovaFluor Blue 725
• NovaFluor Blue 760
• NovaFluor Blue 800
• NovaFluor Red 700
• NovaFluor Red 725
• NovaFluor Red 755
• NovaFluor UV 765
• NovaFluor Violet 690
• NovaFluor Yellow 590
• NovaFluor Yellow 610
• NovaFluor Yellow 660
• NovaFluor Yellow 810
• PE
• PE-Cyanine5
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 645
• Super Bright 702
• Super Bright 780
• Request custom conjugation

Product Specific Information

The O323 monoclonal antibody reacts with human CD27, a lymphocyte-specific member of the TNFR superfamily. CD27 is expressed by a subset of thymocytes and virtually all mature T cells and is upregulated upon T-cell stimulation. CD27 binds to CD70, playing an important role in T cell–B cell interaction.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Pre-diluted and tested by flow cytometry of normal human peripheral blood cells. Recommended usage: 5 µL (0.25 µg) per test (100 µL final volume, 10⁵–10⁸ cells/test).
• Fluorochrome: Brilliant Ultra Violet™ 737 (BUV737), emission at 732 nm, for ultraviolet (355 nm) laser systems.
• Light Sensitivity: Protect from light.
• Fixation: Compatible with IC Fixation Buffer (00-8222) or 1-step Fix/Lyse Solution (00-5333).
• Buffer Recommendation: Use Super Bright Complete Staining Buffer (SB-4401) or Brilliant Stain Buffer (00-4409) to minimize non-specific interactions.
• Fixation Compatibility: Short-term methanol-based fixation is acceptable; avoid long-term methanol exposure.
• Excitation/Emission: 355 nm / 732 nm
• Laser: Ultraviolet laser

BRILLIANT ULTRA VIOLET™ is a trademark of Becton, Dickinson and Company or its affiliates, used under license. Powered by Sirigen™.

Target Information

CD27 is a 50 kDa member of the TNF receptor superfamily, including CD40 and CD30. It regulates cell proliferation and death and is primarily expressed on lymphocytes. CD27 expression increases upon antigen receptor ligation, while CD70 expression requires additional co-stimulatory signals. CD27 forms disulfide-linked homodimers on thymocytes, peripheral T cells, and some B cells. Soluble CD27 (sCD27) is a prognostic marker in B cell malignancies. CD27-binding protein (SIVA) is involved in CD27-mediated apoptosis. Disorders linked to CD27 dysfunction include Lymphoproliferative Syndrome 2 and Autosomal Recessive Lymphoproliferative Syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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