Thermo Fisher Scientific F(ab`)2-Goat anti-Human IgG (H+L) Cross-Adsorbed Secondary Antibody, Biotin
Biotin으로 표지된 염소 유래 anti-Human IgG (H+L) 2차 항체로, ICC/IF, Flow Cytometry, ELISA에 적합. 인간 IgG에 높은 특이성과 교차 반응 최소화. F(ab')2 fragment 형태로 비특이 결합 감소. 연구용으로 신뢰성 높은 검출에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunocytochemistry (ICC/IF) | 1:500–1:20,000 |
| Flow Cytometry (Flow) | 1:500–1:20,000 |
| ELISA | 1:500–1:20,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Gamma Immunoglobins Heavy and Light chains |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 2.5 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.2, with 1% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2535943 |
| Target | IgG |
| Cross Adsorption | Against bovine, mouse and rabbit serum proteins |
| Antibody Form | F(ab`)2 Fragment |
Additional Formats Available
- Alexa Fluor 488
- Alexa Fluor 546
- Alexa Fluor 555
- Alexa Fluor 568
- Alexa Fluor 594
- Alexa Fluor 633
- Alexa Fluor 647
- Alexa Fluor Plus 405
- Alexa Fluor Plus 488
- Alexa Fluor Plus 555
- Alexa Fluor Plus 594
- Alexa Fluor Plus 647
- AP
- FITC
- HRP
- Rhodamine
- TRITC
- Request custom conjugation available
Target Information
Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins. They are useful for detection, sorting, or purification of their specified target. Secondary antibodies enhance versatility and sensitivity by allowing multiple detection systems (e.g., HRP, AP, fluorescence) and signal amplification through multiple secondary bindings to a single primary antibody. These antibodies are typically generated by immunizing the host animal with pooled immunoglobulins from the target species and further purified or modified (e.g., immunoaffinity chromatography, antibody fragmentation, label conjugation) to achieve high specificity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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