Thermo Fisher Scientific F(ab`)2-Goat anti-Human IgG (H+L) Cross-Adsorbed Secondary Antibody, AP
AP 결합 F(ab')2 염소 항-인간 IgG(H+L) 2차 항체로, WB, IHC, ELISA에 적합합니다. 다양한 형광 및 효소 표지 형식으로 제공되며, 교차흡착 처리로 비특이적 결합을 최소화했습니다. 연구용으로 높은 특이성과 신뢰성을 제공합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:20,000 |
| Immunohistochemistry (IHC) | 1:500–1:20,000 |
| ELISA | 1:500–1:20,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Gamma Immunoglobins Heavy and Light chains |
| Conjugate | AP |
| Form | Liquid |
| Concentration | 2.5 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | 30 mM triethanolamine, 1% BSA, 5 mM MgCl₂, 0.1 mM ZnCl₂ |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2535942 |
| Target | IgG |
| Cross Adsorption | Against bovine, mouse and rabbit serum proteins |
| Antibody Form | F(ab')₂ Fragment |
Available Conjugate Formats
- Alexa Fluor 488
- Alexa Fluor 546
- Alexa Fluor 555
- Alexa Fluor 568
- Alexa Fluor 594
- Alexa Fluor 633
- Alexa Fluor 647
- Alexa Fluor Plus 405
- Alexa Fluor Plus 488
- Alexa Fluor Plus 555
- Alexa Fluor Plus 594
- Alexa Fluor Plus 647
- Biotin
- FITC
- HRP
- Rhodamine
- TRITC
- Custom conjugation available upon request
Target Information
Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins. They are useful in the detection, sorting, or purification of their specified targets. Secondary antibodies offer increased versatility, enabling the use of multiple detection systems (e.g., HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification, as multiple secondary antibodies can bind to a single primary antibody. Typically, secondary antibodies are generated by immunizing the host animal with pooled immunoglobulins from the target species and may be further purified or modified (e.g., immunoaffinity chromatography, antibody fragmentation, label conjugation) to produce highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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