Thermo Fisher Scientific F(ab`)2-Goat anti-Human IgG Fc Cross-Adsorbed Secondary Antibody, AP
Goat에서 유래한 F(ab`)2 형태의 anti-Human IgG Fc 2차 항체로, AP로 결합되어 Western blot, IHC, ELISA 등 다양한 응용에 적합합니다. 높은 교차 흡착 특이성과 신호 증폭 효과를 제공합니다. 연구용으로 4°C 또는 -20°C 보관 권장.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:20,000 |
| Immunohistochemistry (IHC) | 1:500–1:20,000 |
| ELISA | 1:500–1:20,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host/Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Gamma Immunoglobin Fc region |
| Conjugate | AP |
| Form | Liquid |
| Concentration | 2.5 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | 1% BSA, 30 mM triethanolamine, 5 mM MgCl₂, 0.1 mM ZnCl₂ |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C (short term), -20°C (long term), avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2535948 |
| Target | IgG Fc |
| Cross Adsorption | Against bovine, horse, mouse, and rabbit serum proteins |
| Antibody Form | F(ab`)₂ Fragment |
Additional Formats Available
- Biotin
- DyLight 488
- DyLight 550
- DyLight 594
- DyLight 650
- DyLight 680
- DyLight 755
- DyLight 800
- FITC
- HRP
- TRITC
- Custom conjugation available upon request
Target Information
Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins. They are used in detection, sorting, or purification of their specific targets. Secondary antibodies provide versatility and enhanced sensitivity through signal amplification, as multiple secondary antibodies can bind to a single primary antibody. These antibodies are typically generated by immunizing the host animal with pooled immunoglobulins from the target species and further purified or modified (e.g., immunoaffinity chromatography, antibody fragmentation, label conjugation) to produce highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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