Thermo Fisher Scientific Phospho-GAB1 (Tyr627) Monoclonal Antibody (T.921.2)

Applications

Tested Application

• Western Blot (WB): 1:1,000 dilution

Product Specifications

Product Specific Information

• MA5-14812 detects Phospho-GAB1 pTyr627 in human samples.
• Predicted to react with mouse and rat based on sequence homology.
• Successfully used in Western blot applications.
• The immunogen is a synthetic phosphopeptide corresponding to residues surrounding Tyr627 of human Gab1.
• Detects Phospho-Gab1 (Tyr627) with a predicted molecular weight of approximately 110 kDa.

Target Information

Gab1 is a 115 kDa multiple docking protein that plays an essential role in cellular growth, transformation, and apoptosis.
It can be phosphorylated by multiple receptor tyrosine kinases (RTKs), including:

• Insulin receptor (IR)
• Platelet-derived growth factor receptor beta (PDGFRβ)
• Hepatocyte growth factor/scatter factor receptor (HGFR/SFR or c-Met)
• Epidermal growth factor receptor (EGF)

Gab1 is tyrosine phosphorylated on at least 16 sites, serving as binding sites for PI3K, Grb2, PLCγ1, Nck, and SHP2.
Phosphorylation at Tyr627 and Tyr659 is critical for SHP2 binding and activation of the ERK/MAPK pathway in response to EGF.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.