Thermo Fisher Scientific PDHA1 Monoclonal Antibody (S.975.6)
PDHA1 단백질을 표적하는 Thermo Fisher Scientific의 단클론 항체로, Western blot, IHC, ICC/IF 등 다양한 응용에 적합합니다. 인간, 생쥐, 설치류 등에서 반응하며 고순도 친화 크로마토그래피로 정제된 액상 항체입니다. 연구용으로만 사용됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000 | - |
| Immunohistochemistry (IHC) | - | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100 | - |
| Immunocytochemistry (ICC/IF) | 1:250 | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Non-human primate, Rat |
| Published Species | Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Monoclonal |
| Type | Antibody |
| Clone | S.975.6 |
| Immunogen | Synthetic peptide corresponding to the sequence of human pyruvate dehydrogenase |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 15 µg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.01M HEPES, pH 7.5, with 0.15M NaCl, 100 µg/mL BSA, 50% glycerol |
| Contains | <0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_10986167 |
Product Specific Information
It is not recommended to aliquot this antibody.
Target Information
The pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial multienzyme complex that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO₂, providing the primary link between glycolysis and the tricarboxylic acid (TCA) cycle.
The PDH complex consists of multiple copies of three enzymatic components:
- Pyruvate dehydrogenase (E1)
- Dihydrolipoamide acetyltransferase (E2)
- Lipoamide dehydrogenase (E3)
The E1 enzyme is a heterotetramer of two alpha and two beta subunits. This gene encodes the E1 alpha 1 subunit containing the E1 active site and plays a key role in PDH complex function.
Mutations in this gene are associated with pyruvate dehydrogenase E1-alpha deficiency and X-linked Leigh syndrome.
Alternatively spliced transcript variants encoding different isoforms have been identified for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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