Thermo Fisher Scientific Phospho-STAT5 alpha (Tyr694) Monoclonal Antibody (J.536.2)
Phospho-STAT5 alpha (Tyr694) 단백질을 검출하는 고특이성 단일클론 항체. Western blot, IHC, ICC/IF, Flow cytometry에 최적화. Human 및 Mouse 시료에 반응하며, 액상 형태로 보관 (-20°C). 연구용으로만 사용 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:800 |
| Immunocytochemistry (ICC/IF) | 1:100–1:400 |
| Flow Cytometry (Flow) | 1:100–1:400 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Monoclonal |
| Type | Antibody |
| Clone | J.536.2 |
| Immunogen | Synthetic peptide corresponding to residues surrounding Tyr694 of Stat5 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 592 µg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.01M HEPES, pH 7.5, with 0.15M NaCl, 100 µg/mL BSA, 50% glycerol |
| Contains | <0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_10980803 |
Product Specific Information
It is not recommended to aliquot this antibody.
Target Information
STAT5 alpha is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by receptor-associated kinases, forming homo- or heterodimers that translocate to the nucleus to act as transcription activators. STAT5 alpha mediates responses to ligands such as IL2, IL3, IL7, GM-CSF, erythropoietin, thrombopoietin, and various growth hormones. Activation of STAT5 alpha in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and essential for tumorigenesis. The mouse counterpart induces expression of BCL2L1/BCL-X(L), suggesting an anti-apoptotic role. STAT5 alpha and STAT5 beta share 96% sequence similarity and are co-expressed in most tissues, with differential mRNA accumulation in muscle and mammary tissue. STAT5 alpha plays critical roles in reproduction, lactation, immune function, and somatic growth.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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