Thermo Fisher Scientific KEAP1 Polyclonal Antibody
Rabbit polyclonal antibody targeting human KEAP1 protein. Validated for WB and IHC applications. Detects 65–70 kDa band in Western blot. Recommended positive control: human lung tissue lysate. Suitable for research use only.
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Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1–2 µg/mL | - |
| Immunohistochemistry (IHC) | - | View 1 publication |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 20 µg/mL | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Not Applicable |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide near the carboxy terminus of human KEAP1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | 0.02% sodium azide |
| Storage Conditions | 2–8°C up to 3 months; for long-term storage, store at -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2551806 |
Product Specific Information
- Suggested positive control: human lung tissue lysate
- Can be used with blocking peptide PEP-1496
- Immunogen located within the last 50 amino acids of KEAP1
- Predicted molecular weight: ~70 kDa
- Observed band in Western blot: 65–70 kDa
- Predicted species reactivity based on immunogen sequence: Pig (100%)
Target Information
KEAP1 encodes a protein containing KELCH-1-like domains and a BTB/POZ domain.
Kelch-like ECH-associated protein 1 interacts with NF-E2-related factor 2 (Nrf2) in a redox-sensitive manner.
Upon dissociation, Nrf2 translocates to the nucleus, inducing expression of the catalytic subunit of gamma-glutamylcysteine synthetase.
Two alternatively spliced transcript variants encoding the same isoform are known for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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