Thermo Fisher Scientific KEAP1 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1-2 µg/mL

-

Immunohistochemistry (IHC)

-

View 1 publication 1 publication

Immunohistochemistry (PFA fixed) (IHC (PFA))

20 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Not Applicable

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A peptide near the carboxy terminus of human KEAP1 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:KEAP1,uniProtId:Q14145-1,ncbiNodeId:9606,antigenRange:624,antigenLength:624,antigenImageFileName:PA5-34454_KEAP1_Q14145-1_Rabbit.svg,antigenImageFileNamePDP:PA5-34454_KEAP1_Q14145-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

0.02% sodium azide

Storage conditions

Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2551806

Product Specific Information

A suggested positive control is human lung tissue lysate.

PA5-34454 can be used with blocking peptide PEP-1496.

The PA5-34454 immunogen is located within the last 50 amino acids of KEAP1.

Predicted molecular ~ 70kD. In Western blot applications, this antibody has been observed to detect a band at: 65-70kD.

Predicted species reactivity based on immunogen sequence: Pig: (100%).

Target Information

This gene encodes a protein containing KELCH-1 like domains, as well as a BTB/POZ domain. Kelch-like ECH-associated protein 1 interacts with NF-E2-related factor 2 in a redox-sensitive manner and the dissociation of the proteins in the cytoplasm is followed by transportation of NF-E2-related factor 2 to the nucleus. This interaction results in the expression of the catalytic subunit of gamma-glutamylcysteine synthetase. Two alternatively spliced transcript variants encoding the same isoform have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.