Thermo Fisher Scientific CD45 Monoclonal Antibody (HI30), Brilliant Ultra Violet 395, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 5 µL (0.125 µg)/test

Product Specifications

Additional Available Formats

• Unconjugated
• Alexa Fluor 532, 561, 647, 660
• APC, APC-eFluor 780
• Biotin
• Brilliant UV 496, 563, 615, 661, 737, 805
• Brilliant Violet 421, 480, 605, 650, 711
• eFluor 450, 506
• FITC
• NovaFluor Blue 510, 585, 690
• NovaFluor Red 710, 725, 755
• NovaFluor Yellow 590, 660, 730, 755
• PE, PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7
• PE-eFluor 610
• PerCP-Cyanine5.5, PerCP-eFluor 710
• Custom conjugation available upon request

Product Specific Information

The HI30 monoclonal antibody reacts with all isoforms of human CD45 (Leukocyte Common Antigen, LCA), expressed on all hematopoietic cells except circulating erythrocytes and platelets. CD45 functions as a tyrosine phosphatase, playing a critical role in lymphocyte activation.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Pre-diluted and validated for normal human peripheral blood cells
• Recommended Use: 5 µL (0.125 µg) per test (in 100 µL final volume, 10⁵–10⁸ cells/test)
• Fluorophore: Brilliant Ultra Violet™ 395 (BUV395), excitation at 350 nm, emission at 395 nm
• Laser Compatibility: Ultraviolet (355 nm) laser

When using multiple polymer dye-conjugated antibodies, use Super Bright Complete Staining Buffer (Cat. SB-4401-42) or Brilliant Stain Buffer™ (Cat. 00-4409-75) to minimize non-specific interactions.

BRILLIANT ULTRA VIOLET™ is a trademark of Becton, Dickinson and Company, used under license. Powered by Sirigen™.

Target Information

CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase expressed on all nucleated hematopoietic cells, constituting up to 10% of lymphocyte surface proteins. It regulates Src-family kinases, T- and B-cell receptor signaling, and cytokine receptor pathways. CD45 isoforms arise via alternative splicing and differ in extracellular domains. It is absent on non-hematopoietic tissues and crucial for lymphocyte development, activation, and survival.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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