Thermo Fisher Scientific CD69 Monoclonal Antibody (FN50), Brilliant Ultra Violet 395, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 5 µL (0.25 µg)/test

Product Specifications

Additional Formats

• Unconjugated
• Alexa Fluor 561
• Alexa Fluor 660
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 615
• Brilliant UV 661
• Brilliant UV 737
• Brilliant UV 805
• Brilliant Violet 421
• Brilliant Violet 480
• Brilliant Violet 650
• Brilliant Violet 711
• Brilliant Violet 786
• eFluor 450
• FITC
• NovaFluor Blue 610-70S
• PE
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 780
• Request custom conjugation

Product Specific Information

The FN50 monoclonal antibody reacts with human CD69, also known as very early activation antigen (VEA). CD69 is approximately 30 kDa and expressed as a disulfide-linked dimer on the cell surface. It is rapidly upregulated upon activation and expressed on lymphocytes, monocytes, and platelets.

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells. Recommended use at 5 µL (0.25 µg) per test (final volume 100 µL). Cell number may vary from 10⁵ to 10⁸ cells/test.

Fluorochrome Information:
Brilliant Ultra Violet™ 395 (BUV395) emits at 395 nm and is intended for cytometers equipped with an ultraviolet (355 nm) laser. Ensure your instrument is compatible with this fluorochrome.

When using multiple polymer dye-conjugated antibodies (Super Bright™, Brilliant Violet™, Brilliant Ultra Violet™), use Super Bright Complete Staining Buffer (SB-4401-42) or Brilliant Stain Buffer™ (00-4409-75) to minimize non-specific interactions.

Excitation: 350 nm
Emission: 395 nm
Laser: Ultraviolet Laser

BRILLIANT ULTRA VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates and used under license. Powered by Sirigen™.

Target Information

CD69 (AIM, Active Inducer Molecule) is a gp28/34 disulfide-bonded homodimer (~60 kDa under non-reducing conditions). It contains one or two N-linked oligosaccharides and is present on activated platelets. In normal peripheral blood, a variable percentage of cells express CD69. It plays a role in lymphocyte signal transduction and may regulate T lymphocyte activation and proliferation. CD69 also transmits signals in natural killer cells and platelets. Alternative splicing results in multiple transcript variants. Diseases associated with CD69 dysfunction include coccidiodomycosis and asthma.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

제품 이미지