Thermo Fisher Scientific CD45RA Monoclonal Antibody (HI100), Brilliant Ultra Violet 395, eBioscience

Thermo Fisher Scientific CD45RA Monoclonal Antibody (HI100), Brilliant Ultra Violet 395, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (1.0 µg)/test

Product Specifications

Additional Formats Available

• Unconjugated
• Alexa Fluor 532
• Alexa Fluor 700
• Alexa Fluor Plus 555
• Alexa Fluor Plus 750
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 496 / 563 / 661 / 737
• Brilliant Violet 421 / 786
• eFluor 450 / 506
• FITC
• NovaFluor Blue, Red, UV, Violet, Yellow series
• PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
• PerCP-Cyanine5.5
• Super Bright 436 / 600 / 645 / 702
• Request custom conjugation available

Product Specific Information

The HI100 monoclonal antibody reacts with human CD45RA, a 220 kDa molecule expressed by subpopulations of CD4+ and CD8+ peripheral T lymphocytes and B cells. CD45RA+ T cell populations are mainly naive/virgin, allowing HI100 mAb to serve as a phenotypic marker to discriminate T cell subsets.

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-diluted and validated for flow cytometry using normal human peripheral blood cells. Recommended use: 5 µL (1.0 µg) per test in a final volume of 100 µL.

Fluorochrome Information:
Brilliant Ultra Violet™ 395 (BUV395) emits at 395 nm and is intended for use on cytometers equipped with an ultraviolet (355 nm) laser. Ensure instrument compatibility before use.

When using multiple polymer dye-conjugated antibodies (Super Bright™, Brilliant Violet™, Brilliant Ultra Violet™), use Super Bright Complete Staining Buffer (Product # SB-4401-42) or Brilliant Stain Buffer™ (Product # 00-4409-75) to minimize non-specific interactions.

Excitation: 350 nm
Emission: 395 nm
Laser: Ultraviolet Laser

BRILLIANT ULTRA VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, used under license. Powered by Sirigen™.

Target Information

CD45RA is an isoform of the CD45 complex with restricted expression among lymphoid cell subtypes. CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase expressed in all nucleated hematopoietic cells, comprising about 10% of lymphocyte surface proteins. It regulates Src-family kinases and is vital for lymphocyte development and antigen signaling.

CD45 isoforms arise from alternative splicing, differing in extracellular domains but sharing transmembrane and cytoplasmic regions. Expression varies by cell type and physiological state. CD45 also modulates integrin-mediated signaling and participates in apoptosis-related DNA fragmentation.

CD45 is expressed on human hematopoietic lineage cells (except mature red cells) and absent in other differentiated tissues. Dysfunction is associated with severe combined immunodeficiency and Hepatitis C.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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