Thermo Fisher Scientific Phospho-Cdc25B (Ser353) Polyclonal Antibody
CDC25B 단백질의 Ser353 인산화 형태를 특이적으로 인식하는 Rabbit Polyclonal 항체. Western blot, IHC, ICC/IF에 적합하며 사람, 생쥐, 영장류 반응성. 고순도 정제 및 안정적 PBS/glycerol 저장 조건 제공.
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Applications
Western Blot (WB)
- Tested Dilution: 1:500–1:2,000
Immunohistochemistry (Paraffin) (IHC (P))
- Tested Dilution: 1:50–1:200
Immunocytochemistry (ICC/IF)
- Tested Dilution: 1:100–1:500
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Non-human primate |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human CDC25B (Accession P30305), corresponding to amino acid residues around phosphorylated Ser353 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage conditions | -20°C |
| Shipping conditions | Wet ice |
| RRID | AB_2816338 |
Product Specific Information
Antibody detects endogenous levels of CDC25B only when phosphorylated at Serine 353.
Target Information
Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Required for G2/M phases of the cell cycle progression and abscission during cytokinesis in an ECT2-dependent manner. Directly dephosphorylates CDK1 and stimulates its kinase activity. The three isoforms seem to have different levels of activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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