Thermo Fisher Scientific CD25 Monoclonal Antibody (PC61.5), Brilliant Violet 421, eBioscience

Thermo Fisher Scientific CD25 Monoclonal Antibody (PC61.5), Brilliant Violet 421, eBioscience

Applications

• Tested Application: Flow Cytometry (Flow)
• Tested Dilution: 0.25 µg/test

Product Specifications

Additional Formats

• Unconjugated
• Alexa Fluor 488, 532, 561, 647, 660, 700
• APC, APC-eFluor 780
• Biotin, Brilliant UV 563, 661, 737, 805
• Brilliant Violet 650, 711, 786
• eFluor 450, 506, FITC
• Functional Grade
• NovaFluor Blue series (585, 610-70S, 660-120S, 760)
• Pacific Orange, PE series (PE, PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7, PE-eFluor 610)
• PerCP series (PerCP-Cyanine5.5, PerCP-eFluor 710)
• Super Bright series (436, 600, 645, 702, 780)
• Custom conjugation available

Product Specific Information

The PC61.5 antibody reacts with mouse CD25, the 55 kDa interleukin-2 receptor alpha chain (IL-2Rα). CD25 is expressed by early progenitors of T and B lineage cells and activated mature lymphocytes. CD25 binds IL-2 with low affinity but associates with CD122 and CD132 to form the high-affinity IL-2 receptor complex. Binding of IL-2 is inhibited by the PC61.5 antibody. CD25 is involved in lymphocyte differentiation and activation.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Mouse splenocytes (≤0.25 µg/test, 100 µL final volume)
• Recommended: Empirical titration for optimal performance

Brilliant Violet™ 421 (BV421) emits at 423 nm and is designed for cytometers with a violet (405 nm) laser.
When using multiple polymer dye-conjugated antibodies, use Super Bright Complete Staining Buffer (SB-4401-42) or Brilliant Stain Buffer™ (00-4409-75) to minimize non-specific interactions.

• Excitation: 407 nm
• Emission: 423 nm
• Laser: Violet Laser

BRILLIANT VIOLET™ is a trademark of Becton, Dickinson and Company and used under license. Powered by Sirigen™.

Target Information

CD25 (IL2RA) encodes the interleukin-2 receptor alpha chain, part of the heterotrimeric IL-2 receptor complex with IL2RB and IL2RG. This receptor regulates T and B cell proliferation, differentiation, and immune response. IL2 acts as a growth and differentiation factor and regulates apoptosis. Mutations in IL2RA are linked to immunological disorders such as interleukin-2 receptor alpha deficiency.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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