Thermo Fisher Scientific CD25 Monoclonal Antibody (PC61.5), Brilliant Violet 421, eBioscience
Mouse CD25 단백질을 인식하는 Rat IgG1 단클론 항체로, Brilliant Violet 421 형광체가 결합되어 있습니다. Flow Cytometry용으로 최적화되어 있으며, 높은 특이성과 안정성을 제공합니다. 4°C 보관, 비동결 조건에서 유지됩니다.
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Thermo Fisher Scientific CD25 Monoclonal Antibody (PC61.5), Brilliant Violet 421, eBioscience
Applications
- Tested Application: Flow Cytometry (Flow)
- Tested Dilution: 0.25 µg/test
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Host / Isotype | Rat / IgG1, lambda |
| Recommended Isotype Control | Rat IgG1 kappa Isotype Control (eBRG1), Brilliant Violet™ 421, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | PC61.5 |
| Conjugate | Brilliant Violet™ 421 |
| Excitation / Emission Max | 406 / 423 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2925498 |
Additional Formats
- Unconjugated
- Alexa Fluor 488, 532, 561, 647, 660, 700
- APC, APC-eFluor 780
- Biotin, Brilliant UV 563, 661, 737, 805
- Brilliant Violet 650, 711, 786
- eFluor 450, 506, FITC
- Functional Grade
- NovaFluor Blue series (585, 610-70S, 660-120S, 760)
- Pacific Orange, PE series (PE, PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7, PE-eFluor 610)
- PerCP series (PerCP-Cyanine5.5, PerCP-eFluor 710)
- Super Bright series (436, 600, 645, 702, 780)
- Custom conjugation available
Product Specific Information
The PC61.5 antibody reacts with mouse CD25, the 55 kDa interleukin-2 receptor alpha chain (IL-2Rα). CD25 is expressed by early progenitors of T and B lineage cells and activated mature lymphocytes. CD25 binds IL-2 with low affinity but associates with CD122 and CD132 to form the high-affinity IL-2 receptor complex. Binding of IL-2 is inhibited by the PC61.5 antibody. CD25 is involved in lymphocyte differentiation and activation.
- Applications Reported: Flow cytometric analysis
- Applications Tested: Mouse splenocytes (≤0.25 µg/test, 100 µL final volume)
- Recommended: Empirical titration for optimal performance
Brilliant Violet™ 421 (BV421) emits at 423 nm and is designed for cytometers with a violet (405 nm) laser.
When using multiple polymer dye-conjugated antibodies, use Super Bright Complete Staining Buffer (SB-4401-42) or Brilliant Stain Buffer™ (00-4409-75) to minimize non-specific interactions.
- Excitation: 407 nm
- Emission: 423 nm
- Laser: Violet Laser
BRILLIANT VIOLET™ is a trademark of Becton, Dickinson and Company and used under license. Powered by Sirigen™.
Target Information
CD25 (IL2RA) encodes the interleukin-2 receptor alpha chain, part of the heterotrimeric IL-2 receptor complex with IL2RB and IL2RG. This receptor regulates T and B cell proliferation, differentiation, and immune response. IL2 acts as a growth and differentiation factor and regulates apoptosis. Mutations in IL2RA are linked to immunological disorders such as interleukin-2 receptor alpha deficiency.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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