Thermo Fisher Scientific CD38 Monoclonal Antibody (HIT2), Brilliant Violet 421, eBioscience

Applications

• Flow Cytometry (Flow)
  Tested Dilution: 5 µL (0.5 µg)/test

Product Specifications

Additional Formats

• Unconjugated
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 805
• Brilliant Violet 480
• eFluor 450
• FITC
• PE
• PE-Cyanine5
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 702
• Request custom conjugation

Product Specific Information

The HIT2 monoclonal antibody reacts with the human CD38 molecule, an approximately 45 kDa type II transmembrane protein. CD38 is expressed by thymocytes, peripheral B cells including plasma cells, activated T cells, and monocytes. CD38 acts as a counter-receptor for CD31 and exhibits cyclase and hydrolase enzymatic activities.

Applications Reported: Flow cytometric analysis.
Applications Tested: Pre-diluted and validated for flow cytometric analysis of normal human peripheral blood cells. Recommended usage: 5 µL (0.5 µg) per 100 µL test volume (10⁵–10⁸ cells/test).

Fluorochrome Information:
Brilliant Violet™ 421 (BV421) emits at 423 nm and is designed for cytometers equipped with a violet (405 nm) laser. Ensure instrument compatibility before use.

Buffer Recommendations:
When using multiple polymer dye-conjugated antibodies (e.g., Super Bright™, Brilliant Violet™, Brilliant Ultra Violet™), it is recommended to use:

• Super Bright Complete Staining Buffer (Product # SB-4401-42)
• Brilliant Stain Buffer™ (Product # 00-4409-75)

Excitation: 407 nm
Emission: 423 nm
Laser: Violet laser

BRILLIANT VIOLET™ is a trademark of Becton, Dickinson and Company or its affiliates, used under license. Powered by Sirigen™.

Target Information

CD38 (NAD⁺ glycohydrolase) is a type II transmembrane glycoprotein involved in activation, proliferation, and differentiation of lymphocytes, and mediates apoptosis of myeloid and lymphoid progenitor cells. It functions as a multi-catalytic ectoenzyme (ADP-ribosyl cyclase, cyclic ADP-ribose hydrolase, NAD⁺ glycohydrolase) and as a cell surface receptor.

Antibodies to CD38 are useful for:

• Subtyping lymphomas and leukemias
• Detecting plasma cells (e.g., myeloma identification)
• Marking activated B and T cells

CD38 is expressed in pancreas, liver, kidney, malignant lymphoma, and neuroblastoma.
Diseases associated with CD38 dysfunction include chronic lymphocytic leukemia and Richter’s Syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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