Thermo Fisher Scientific TLR4 Monoclonal Antibody (HTA125), FITC
TLR4 단일클론 항체(HTA125), FITC 형광 표지로 인간, 마우스, 개 시료에 반응. IHC, ICC/IF, Flow Cytometry에 적합하며 단핵세포의 LPS 활성화를 억제 가능. 단백질 G 정제, 4°C 암소 저장.
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Thermo Fisher Scientific TLR4 Monoclonal Antibody (HTA125), FITC
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (IHC) | Assay-Dependent |
| Immunocytochemistry (ICC/IF) | Assay-Dependent |
| Flow Cytometry (Flow) | 1 µL / 1 million cells |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Dog, Human, Mouse |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | HTA125 |
| Immunogen | Ba/F3 cell line expressing human TLR4 cell surface antigen |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 0.95 mg/mL |
| Purification | Protein G |
| Storage Buffer | 50 mM sodium borate |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2537726 |
Additional Formats
Product Specific Information
This antibody has been demonstrated to block activation of monocytes with LPS.
Target Information
TLR4 is a member of the Toll-like receptor (TLR) family, playing a key role in pathogen recognition and activation of innate immunity. TLRs are conserved across species and are structurally and functionally similar.
TLR4 is most abundantly expressed in placenta and in the myelomonocytic subpopulation of leukocytes. It forms part of the LPS receptor complex with CD14 and LY96, mediating signal transduction upon recognition of lipopolysaccharide from gram-negative bacteria.
Mutations in the TLR4 gene influence LPS responsiveness, and multiple transcript variants exist. TLR4 expression is seen in peripheral blood monocytes, some B-cells, and placenta. TLR4-deficient mice show increased susceptibility to Gram-negative sepsis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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