Thermo Fisher Scientific TLR4 Monoclonal Antibody (HTA125)
TLR4 단백질을 인식하는 HTA125 클론의 마우스 단일클론 항체. Western blot, IHC, ICC/IF, Flow cytometry 등 다양한 응용 가능. 단백질 G 정제, PBS buffer에 0.02% sodium azide 포함. 연구용으로만 사용.
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Thermo Fisher Scientific TLR4 Monoclonal Antibody (HTA125)
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1–2 µg/mL | - |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-Dependent | - |
| Immunocytochemistry (ICC/IF) | Assay-Dependent | - |
| Flow Cytometry (Flow) | 0.25 µg × 10⁶ cells | 3 publications |
| Immunoprecipitation (IP) | 1:10–1:500 | - |
| Neutralization (Neu) | Assay-Dependent | 1 publication |
| Functional Assay | Assay-Dependent | - |
| In vitro Assay (IV) | Assay-Dependent | - |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Dog, Human, Mouse |
| Published Species | Human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | HTA125 |
| Immunogen | Ba/F3 cell line expressing human TLR4 cell surface antigen |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term; for long-term storage, store at -20°C avoiding freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2537723 |
Additional Formats
Target Information
TLR4 is a member of the Toll-like receptor (TLR) family, playing a key role in pathogen recognition and activation of innate immunity. TLRs are conserved across species and share structural and functional similarities. TLR4 is highly expressed in placenta and myelomonocytic leukocytes. It mediates cellular responses to lipopolysaccharides (LPS) from Gram-negative bacteria and forms a receptor complex with CD14 and LY96. This receptor facilitates recognition of pathogen-associated molecular patterns (PAMPs) and triggers cytokine production for immune defense. Mutations in TLR4 can affect LPS responsiveness. TLR4 is expressed by monocytes, a subset of B-cells, and placenta. TLR4-deficient mice show increased susceptibility to Gram-negative sepsis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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배송/결제/교환/반품 안내
배송 정보
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