Thermo Fisher Scientific Histone H3 Polyclonal Antibody
Western blot, IHC, IP에 사용 가능한 Histone H3 폴리클로날 항체로 인간, 마우스, 랫트에 반응합니다. 항원 친화 크로마토그래피로 정제되었으며 핵 내 히스톤 단백질 검출에 적합합니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–1 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 2–10 µg/mL |
| Immunoprecipitation (IP) | 2–5 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic peptide GVKKP-H-RYKPG of human histone H3 (amino acids 35–46). |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with proprietary stabilizer |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Positive control: 3T3 Nuclear Extract
- Cellular location: Nucleus
Target Information
Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3, along with four core histone proteins, binds to DNA forming the structure of the nucleosome. Histones play a central role in transcription regulation, DNA repair, DNA replication, and chromosomal stability. Post-translational modifications of histones can alter chromatin structure or facilitate the binding of transcription factors. The N-terminal tail of histone H3 can undergo methylation, acetylation, and phosphorylation, influencing various cellular processes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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