Thermo Fisher Scientific CD39 Monoclonal Antibody (eBioA1 (A1)), Super Bright 600, eBioscience

Thermo Fisher Scientific CD39 Monoclonal Antibody (eBioA1 (A1)), Super Bright 600, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 5 µL (0.25 µg)/test
• View 6 publications

Product Specifications

Available Formats

• Unconjugated
• APC
• APC-eFluor 780
• Brilliant UV 805
• FITC
• NovaFluor Blue 610-70S
• NovaFluor Blue 660-120S
• NovaFluor Blue 690
• NovaFluor Red 700
• NovaFluor Red 725
• NovaFluor Red 755
• NovaFluor Yellow 660
• NovaFluor Yellow 755
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 702
• Request custom conjugation

Product Specific Information

The eBioA1 monoclonal antibody reacts with human CD39, also known as ectonucleoside triphosphate diphosphohydrolase 1 (ENTPD1) or NTPDase.
CD39 is an integral membrane protein with two transmembrane domains and exists as a homotetramer. It is a key ectoenzyme of the immune system that removes toxic extracellular ATP by converting it to ADP or AMP. CD39 works together with CD73 to hydrolyze ATP and is well characterized on Langerhans cells.

Expression of CD39 was initially identified on activated lymphocytes and is also found on subsets of T cells, B cells, dendritic cells, and weakly on monocytes and granulocytes.
Recently, CD39 and CD73 have been found on regulatory T cells (Treg), aiding their entry into inflamed areas with high ATP levels. Expression on Foxp3+CD4+ cells ranges from 25–45%.

Applications Reported

This antibody is reported for use in flow cytometric analysis.

Applications Tested

Pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. Use at 5 µL (0.25 µg) per test (100 µL final volume). Cell number may range from 10⁵ to 10⁸ cells/test. Titrate for optimal performance.

Fluorochrome Information

Super Bright 600 is a tandem dye excited with the violet laser (405 nm) and emits at 600 nm. Recommended filter: 610/20 bandpass.
Use Super Bright Complete Staining Buffer (Product # SB-4401) when using multiple Super Bright conjugates to minimize non-specific interactions.

Handling and Storage

• Light sensitivity: Protect from light to prevent photo-induced oxidation.
• Fixation: Samples can be stored up to 3 days at 2–8°C in the dark using IC Fixation Buffer (Product # 00-8222) or 1-step Fix/Lyse Solution (Product # 00-5333).
• Excitation: 405 nm
• Emission: 600 nm
• Laser: Violet

Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

Target Information

CD39 cell surface antigen (70–100 kDa) is expressed on peripheral blood B cells, monocytes, T cell clones, and weakly on granulocytes.
It has intrinsic ecto-ATPase activity and is induced on T cells and upregulated on B cells as a late activation antigen.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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