Thermo Fisher Scientific Phospho-Raf-A (Ser186) Polyclonal Antibody
Ser186 위치의 인산화 Raf-A 단백질을 인식하는 폴리클로날 항체로, WB 및 IHC(P) 분석에 적합합니다. Rabbit IgG 호스트 기반이며, 항원 친화 크로마토그래피로 정제되었습니다. 세포막, 세포질, 핵 내 Raf-A 검출에 유용합니다.
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Thermo Fisher Scientific Phospho-Raf-A (Ser186) Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–1 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 2–10 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Bovine, Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide TPQGP-[S]-PRT containing phosphorylation site Ser186 of human Raf-A protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | 25 mM Tris with proprietary stabilizer |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Positive control: Human cervix carcinoma
- Cellular location: Cell membrane, cytoplasm, nucleus
Target Information
Several serine/threonine protein kinases are intermediates in signal transduction pathways, including ERK/MAP kinases, ribosomal S6 kinase (Rsk), and Raf-1.
Raf-1 is a cytoplasmic protein with intrinsic serine/threonine activity, broadly expressed in nearly all tested cell lines. It is the cellular homolog of v-Raf, the transforming gene product of the 3611 strain of murine sarcoma virus.
Unregulated kinase activity of v-Raf is associated with transformation and mitogenesis, while Raf-1 activity is normally suppressed by a regulatory N-terminal domain.
A-Raf, another member of the Raf gene family, shows substantial homology to Raf-1 within the kinase domain but less elsewhere. Expression of A-Raf is highest in urogenital tissues and kidney, and lowest in brain tissue.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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