Thermo Fisher Scientific CD3 Monoclonal Antibody (OKT3), Super Bright 436, eBioscience
인간 CD3 복합체의 epsilon 서브유닛을 인식하는 OKT3 단클론 항체. Flow cytometry에 최적화되어 있으며 Super Bright 436 형광체로 결합. 면역억제 연구 및 T세포 활성화 분석에 활용 가능. 405 nm 레이저로 여기, 436 nm에서 방출.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 1 publication |
| Immunocytochemistry (ICC/IF) | - | View 1 publication |
| Flow Cytometry (Flow) | 5 µL (0.5 µg)/test | View 12 publications |
| Functional Assay | - | View 1 publication |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Mouse / IgG2a, kappa |
| Recommended Isotype Control | Mouse IgG2a kappa Isotype Control (eBM2a), Super Bright™ 436, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OKT3 |
| Conjugate | Super Bright™ 436 |
| Excitation / Emission Max | 413 / 431 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2734957 |
Available Formats
- Unconjugated
- Alexa Fluor 488, Alexa Fluor 700
- APC, APC-eFluor 780
- Biotin
- Brilliant UV 395, 563, 615, 661, 737, 805
- Brilliant Violet 421, 605
- eFluor 450, 506, 660
- FITC
- Functional Grade
- NovaFluor Blue 510, 585
- NovaFluor Yellow 590, 660, 730
- PE, PE-Cyanine7, PE-eFluor 610
- PerCP-Cyanine5.5, PerCP-eFluor 710
- Super Bright 600, 645, 702, 780
- Custom conjugation available
Product Specific Information
The OKT3 monoclonal antibody recognizes an epitope on the epsilon-subunit within the human CD3 complex. It has potent immunosuppressive properties in vivo and has been effective in treating renal, heart, and liver allograft rejection.
CD3 subunits (gamma, delta, epsilon) are essential for TCR complex assembly, trafficking, and surface expression. CD3 is expressed by thymocytes and mature T cells. Crosslinking of TCR triggers intracellular signaling leading to activation and proliferation.
- Applications Reported: Flow cytometric analysis
- Applications Tested: Pre-titrated and tested by flow cytometry of normal human peripheral blood cells
- Recommended Use: 5 µL (0.5 µg) per test in 100 µL final volume
- Excitation/Emission: 405 nm / 436 nm (Violet laser)
- Recommended Filter: 450/50 bandpass or equivalent
When using multiple Super Bright dye-conjugated antibodies, use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize non-specific interactions.
Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Target Information
The CD3 complex transduces antigen-recognition signals into T cells and regulates TCR expression. It includes CD3 gamma, delta, epsilon, and zeta subunits, all members of the immunoglobulin superfamily encoded on chromosome 11.
- CD3 is expressed on 68–82% of normal peripheral blood lymphocytes and 65–85% of thymocytes.
- It is absent on B or NK cells.
- Defects in CD3 genes are associated with CD3 immunodeficiency.
- Plays roles in TCR-induced growth arrest, survival, and proliferation.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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