Thermo Fisher Scientific 14-3-3 gamma Monoclonal Antibody (KC21)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:3,000

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

1:500

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Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

KC21

Immunogen

N-terminal fragment of human 14-3-3 gamma where the N-terminal Val was acetylated. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:14-3-3 gamma,uniProtId:P61981-1,ncbiNodeId:9606,antigenRange:1,antigenLength:247,antigenImageFileName:MA1-16587_14-3-3_gamma_P61981-1_House_mouse.svg,antigenImageFileNamePDP:MA1-16587_14-3-3_gamma_P61981-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_568418

Product Specific Information

Suggested positive control: antigen standard for YWHAG (transient overexpression lysate), HeLa whole cell extract.

Target Information

14-3-3 gamma encodes a product that belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the rat ortholog. It is induced by growth factors in human vascular smooth muscle cells, and is also highly expressed in skeletal and heart muscles, suggesting an important role for this protein in muscle tissue. It has been shown to interact with RAF1 and protein kinase C, proteins involved in various signal transduction pathways.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.