Thermo Fisher Scientific RAD21 Monoclonal Antibody (CM110-2C10)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500

-

Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

CM110-2C10

Immunogen

N-terminus fusion protein of the C-terminal fragment (residues 544-631) of the Scc1 subunit of human cohesin. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:RAD21,uniProtId:O60216-1,ncbiNodeId:9606,antigenRange:544-631,antigenLength:631,antigenImageFileName:MA1-16585_RAD21_O60216-1_House_mouse.svg,antigenImageFileNamePDP:MA1-16585_RAD21_O60216-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

0.1% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_568497

Product Specific Information

In Western blot, two bands are seen: the full-length Rad21 is seen around 130 kDa, while the cleaved Rad21 is seen around 70 kDa.

Suggested positive control: antigen standard for RAD21 (transient overexpression lysate), Hela whole cell extract.

Target Information

This gene encodes a nuclear protein that regulates transcriptional elongation and pre-mRNA splicing. The encoded protein interacts with the hyperphosphorylated C-terminal domain of RNA polymerase II via multiple FF domains, and with the pre-mRNA splicing factor SF1 via a WW domain. Alternative splicing results in multiple transcripts variants encoding different isoforms.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.