
Thermo Fisher Scientific CD66 Monoclonal Antibody (CD66a-B1.1), Super Bright 436, eBioscience
CD66a-B1.1 단클론 항체로 인간 CD66a, c, d, e 인식. Super Bright 436 형광체 결합으로 유세포분석(Flow Cytometry)에 최적화. 405 nm 레이저에서 여기, 436 nm에서 방출. 암세포 및 면역세포 표지 연구용.
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Thermo Fisher Scientific CD66 Monoclonal Antibody (CD66a-B1.1), Super Bright 436, eBioscience
Applications
- Flow Cytometry (Flow)
Tested Dilution: 5 µL (0.25 µg)/test
Publications: View 1 publication
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Not Applicable |
| Host / Isotype | Mouse / IgG2a, kappa |
| Recommended Isotype Control | Mouse IgG2a kappa Isotype Control (eBM2a), Super Bright™ 436, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | CD66a-B1.1 |
| Conjugate | Super Bright™ 436 |
| Excitation/Emission Max | 413 / 431 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2762520 |
Additional Formats
Product Specific Information
The CD66a-B1.1 monoclonal antibody reacts with human CD66a, c, d, and e (CEACAM1, CEACAM6, CEACAM3, and CEA).
CD66 is expressed on endothelial and epithelial cells, as well as neutrophils and monocytes. Myeloma and cancer cells also express CD66. Expression can be induced on T cells, B cells, and CD16-negative NK cells.
CD66 variants differ by cytoplasmic domain length containing ITIM motifs involved in inhibitory signaling. It plays roles in cell adhesion, growth, neutrophil activation, and signaling. Variants interact homo- and heterotypically.
Applications Reported: Flow cytometric analysis.
Applications Tested: Pre-titrated and validated for flow cytometry on normal human peripheral blood cells at 5 µL (0.25 µg)/test (100 µL final volume, 10⁵–10⁸ cells/test).
Super Bright 436 is excited at 405 nm and emits at 436 nm. Recommended filter: 450/50 bandpass or equivalent.
Use Super Bright Complete Staining Buffer (Product # SB-4401) when using multiple Super Bright dyes to minimize non-specific polymer interactions.
Excitation: 405 nm
Emission: 436 nm
Laser: Violet Laser
Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Target Information
CD66 molecules (180–200 kDa glycoproteins) belong to the carcinoembryonic antigen family. Present on blood granulocytes and some tissue macrophages; absent from other hematopoietic cells. Expression increases upon granulocyte activation.
CD66a (CEACAM1) and CD66d (CEACAM3), as well as CEACAM2 and 4, are transmembrane proteins.
CD66c (CEACAM6) and CD66e (CEACAM5), as well as CEACAM7 and 8, are GPI-anchored membrane proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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