Thermo Fisher Scientific LPAR4 Polyclonal Antibody

Applications

Immunohistochemistry (Paraffin) [IHC (P)]

• Tested Dilution: 10–20 µg/mL

Product Specifications

Product Specific Information

• Percent identity with other species by BLAST analysis:
  • Human, Gorilla (100%)
  • Gibbon, Marmoset, Mouse, Rat, Bat, Hamster, Rabbit, Pig (94%)
  • Monkey, Bovine, Panda, Horse (88%)
  • Dog, Elephant (82%)
• For IHC(P), use heat-induced antigen retrieval in pH 6.0 citrate buffer.
• After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.

Target Information

EDG4 belongs to a family of G-protein coupled receptors whose ligands are lysophospholipids. There are eight known members of the EDG receptor family, implicated in mediating growth-related effects such as:

• Induction of cellular proliferation
• Alterations in differentiation and survival
• Suppression of apoptosis

They also evoke cellular effector functions dependent on cytoskeletal responses such as contraction, secretion, adhesion, and chemotaxis.
EDG receptors are developmentally regulated and differ in tissue distribution.
They couple to multiple types of G proteins to signal through ras and MAP kinase, rho, phospholipase C, and several protein tyrosine kinases.
EDG4 is expressed in testes, ovarian tumor, and leukocyte-containing tissues.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.