Thermo Fisher Scientific ITGB1 Polyclonal Antibody
Human 및 비인간 영장류 반응성 ITGB1 폴리클로날 항체로, WB·IHC·ICC/IF에 적합. 항원 친화 크로마토그래피로 정제된 고순도 제품. 세포 부착 및 면역세포 이동 연구에 활용 가능. PBS 버퍼에 0.1% sodium azide 포함, 장기 보관 시 -20°C 권장.
- 카탈로그번호
- PA532887
- 판매단위
- pk
카탈로그
1개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific ITGB1 Polyclonal Antibody
Thermo Fisher Scientific ITGB1 Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 2.5–5 µg/mL |
| Immunocytochemistry (ICC/IF) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Non-human primate |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic 17 amino acid peptide from extracellular domain of human Integrin Beta 1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | 0.1% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2550348 |
Product Specific Information
- Percent identity with other species by BLAST analysis:
Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset (100%); Dog, Bat, Panda, Horse (94%); Sheep, Bovine, Cat, Elephant, Pig (88%) - For IHC(P), use heat-induced antigen retrieval in pH 6.0 citrate buffer.
After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.
Target Information
ITGB1 (Integrin Subunit Beta 1, beta1 integrin subunit, GPIIa, CD29) is a 110 kDa cell surface glycoprotein widely expressed by various cells including all leukocytes. ITGB1 forms non-covalently linked heterodimers with at least six different alpha chains (α1–α6, CDa–f), determining the binding properties of beta1 (VLA) integrins.
ITGB1 functions as a cell adhesion molecule on platelets and as a component of protein complexes binding to extracellular matrix proteins. Decreased expression correlates with multidrug resistance in tumor cells. It is up-regulated in leukocytes during migration and plays a critical role in immune cell trafficking.
Additionally, ITGB1 regulates tissue damage and inflammatory responses such as those in inflammatory bowel disease. Through ITGB1-dependent mechanisms, fibronectin and type I collagen enhance cytokine secretion of human airway smooth muscle in response to IL-1β.
More than eight beta subunits with numerous splice variants exist in mammals. Two major forms—beta1A and beta1D—differ by 13 amino acids and exhibit mutually exclusive tissue distribution: beta1A in all tissues except cardiac and skeletal muscle, which express beta1D.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.