Thermo Fisher Scientific GeneChip Human Gene 2.1 ST Array Plate, 1 x 16 array plate

Applied Biosystems™ GeneChip™ Human Gene 2.1 ST Array Plate

The Human Gene 2.1 ST 16-Array Plate and Trays provide accurate, sensitive, and comprehensive measurement of protein-coding and long intergenic non-coding RNA (lincRNA) transcripts.

Comprehensive Design

To match the research community’s understanding of the transcriptome, these whole-transcript arrays include probes for both messenger RNA (mRNA) and long intergenic non-coding RNA (lincRNA). This design enables a complete expression profile of mRNA and intermediary lincRNA transcripts that influence mRNA expression.

Research Background

Over the past two decades, research has focused on protein-coding mRNA and its role in cellular processes such as disease and development. Recently, more than 10,000 transcripts (>200 bases) with little or no protein-coding potential have been identified. Although few of these non-coding RNAs have functional annotations, evidence shows that differential expression of lincRNAs plays a key role in disease progression and cancer.
Advancements in transcriptome profiling have revealed lincRNAs’ involvement in diverse cellular functions:

• Regulation of mRNA transcription
• Regulation of mRNA post-transcriptional modifications
• Occlusion or recruitment of transcription factor binding
• Activation and transport of transcription factors
• Interaction with accessory proteins
• Guiding protein complexes to genomic locations

Key Benefits

• Comprehensive coverage to discover novel biological insights

  • 30,000 coding transcripts

  • 11,000 long intergenic non-coding transcripts
• High reproducibility (Intra-lot correlation coefficient = 0.99)

Content Profile

Since the design of the Human Gene 1.1 ST Array Plates, numerous new lincRNAs have been identified. The Human Gene 2.1 ST Array Plates were developed to measure differential expression of this expanding RNA class.
To enhance lincRNA data from RefSeq, sequences and transcripts were integrated from:

• lncRNA db
• Broad Institute Human Body Map lincRNAs and TUCP catalog (link)

제품 사양