Thermo Fisher Scientific GeneChip Zebrafish Gene 1.0 ST Array, 30 arrays

Applied Biosystems™ GeneChip™ Zebrafish Gene 1.0 ST Array

The GeneChip™ Zebrafish Gene 1.0 ST Arrays enable you to:

• Measure expression across the entire gene with higher resolution and accuracy than classical 3'-biased microarray solutions
• Obtain accurate and reproducible data using multiple independent measurements for each transcript

Background

Model and applied research organisms are essential for comparative genomics, evolutionary biology, and for understanding molecular mechanisms underlying human disease and agricultural improvement.
The GeneChip Gene 1.0 ST Arrays were developed for a wide range of model organisms and offer whole-transcript coverage.
Design collaborations include:

• Alan Archibald (Roslin Institute, Porcine Array)
• Leonard Zon and Yi Zhou (Children’s Hospital Boston & Harvard Medical School, Zebrafish Array)

Key Benefits

• Highest transcript coverage: Up to 26 probes per transcript for confident expression measurement
• Whole-transcriptome analysis: Detect isoforms missed by 3'-biased designs
• High data correlation: Inter- and intra-array correlation (R > 0.99)

Proven Performance

GeneChip Gene 1.0 ST Arrays provide whole-transcriptome coverage based on the latest genomic content.
This design ensures accurate detection and higher resolution than classical 3'-biased arrays, enabling identification of splice variants, non-polyadenylated transcripts, alternative polyadenylation, and truncated transcripts.

Complete Solution

For optimal performance, the Gene 1.0 ST Array is part of a comprehensive system including:

• GeneChip WT Sense Target Labeling and Control Reagents
• GeneChip Fluidics Station 450 for automated array processing
• GeneChip Scanner 3000 7G (optional Autoloader) for image acquisition

Data Analysis Tools

• Expression Console Software: Easy probe set summarization and data quality evaluation
• NetAffx Analysis Center: Comprehensive probe and annotation database with flexible query tools
• Integrated Genome Browser (IGB): Visualize genomic annotations (RefSeq, SNPs, locations) alongside expression data

Workflow

Gene 1.0 ST Array workflow follows standard 3'-expression analysis protocols using Expression Console, third-party GeneChip-compatible software, and NetAffx/IGB annotation tools.

Content Profile

GeneChip Gene 1.0 ST Arrays interrogate up to 26 unique sequences per transcript (25-mer probes, ~650 bases).
This broad probe coverage ensures superior performance, data confidence, and adaptability as genomic knowledge evolves.

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