Takara T4 DNA Polymerase

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Applications

• Generation of blunt double-stranded DNA from DNA containing 5/3 overhangs
• 5-end or 3-end labeling of double-stranded DNA
• In vitro mutagenesis

Buffer

Supplied with 10X Reaction Buffer [330 mM Tris-acetate (pH 7.9), 660 mM potassium acetate, 100 mM magnesium acetate and 5 mM DTT] and 0.1% BSA.

Product citations

Butler, E. T. & Chamberlins, M. J. Bacteriophage SP6-specific RNA Polymerase I. ISOLATION AND CHARACTERIZATION OF THE ENZYME*. J. Biol. Chem. 257, 5772–5778 (1982).

Green, M. R., Maniatis, T. & Melton, D. A. Human β-globin pre-mRNA synthesized in vitro is accurately spliced in Xenopus oocyte nuclei. Cell 32, 681–694 (1983).

Kotaoi, H., Ishizalci, Y., Hiraoka, N. & Obayashi, A. Nucleotide sequence and expression of the doned gene of bacteriophage SP6 RNA polymerase. Nucleic Acids Res. 15, (1987).

Melton, D. A. et al. Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter. Nucleic Acids Res. 12, 7035–56 (1984).

Pelletier, J. & Sonenberg, N. Insertion mutagenesis to increase secondary structure within the 5′ noncoding region of a eukaryotic mRNA reduces translational efficiency. Cell 40, 515–526 (1985).

Sagawa, H., Ohshima, A. & Kato, I. A tightly regulated expression system in Escherichia coli with SP6 RNA polymerase. Gene 168, (1996).

Schenborn, E. T. & Mierendorf, R. C. A novel transcription property of SP6 and T7 RNA polymerases: dependence on template structure. Nucleic Acids Res. 13, 6223–36 (1985).

Additional product information

Please see the product`s Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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