Takara T4 DNA Polymerase

Takara T4 DNA Polymerase

Applications

• Generation of blunt double-stranded DNA from DNA containing 5′/3′ overhangs
• 5′-end or 3′-end labeling of double-stranded DNA
• In vitro mutagenesis

Buffer Composition

Supplied with 10X Reaction Buffer containing:

• 330 mM Tris-acetate (pH 7.9)
• 660 mM potassium acetate
• 100 mM magnesium acetate
• 5 mM DTT
• 0.1% BSA

Product Citations

• Butler, E. T. & Chamberlins, M. J. J. Biol. Chem. 257, 5772–5778 (1982).
• Green, M. R., Maniatis, T. & Melton, D. A. Cell 32, 681–694 (1983).
• Kotaoi, H., Ishizalci, Y., Hiraoka, N. & Obayashi, A. Nucleic Acids Res. 15 (1987).
• Melton, D. A. et al. Nucleic Acids Res. 12, 7035–7056 (1984).
• Pelletier, J. & Sonenberg, N. Cell 40, 515–526 (1985).
• Sagawa, H., Ohshima, A. & Kato, I. Gene 168 (1996).
• Schenborn, E. T. & Mierendorf, R. C. Nucleic Acids Res. 13, 6223–6236 (1985).

Additional Product Information

Please refer to the product’s Certificate of Analysis for details on storage conditions, components, and technical specifications.
Kit Components List and Certificates of Analysis are available under the Documents tab.

Supplier Contact Information

Takara Bio USA, Inc.
United States/Canada: +1.800.662.2566
Asia Pacific: +1.650.919.7300
Europe: +33.(0)1.3904.6880
Japan: +81.(0)77.565.6999

For Research Use Only. Not for use in diagnostic procedures.
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