Thermo Fisher Scientific CCL5 (RANTES) Polyclonal Antibody, PeproTech
Thermo Fisher Scientific의 CCL5 (RANTES) Polyclonal Antibody는 인간 RANTES 단백질을 표적으로 하는 토끼 유래 항체로, Western blot, ELISA 등 다양한 분석에 적합합니다. 고순도 항원 친화 크로마토그래피로 정제되어 높은 특이성과 감도를 제공합니다.
- 카탈로그번호
- 500-P36-xxx (3개 옵션)
- 판매단위
- pk
카탈로그
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Thermo Fisher Scientific CCL5 (RANTES) Polyclonal Antibody, PeproTech
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.1–0.2 µg/mL | View 2 publications |
| Immunocytochemistry (ICC/IF) | – | View 1 publication |
| ELISA | 0.5–2.0 µg/mL | View 2 publications |
| Neutralization (Neu) | – | View 2 publications |
| In vitro Assay (IV) | – | View 1 publication |
| Immunostaining (IS) | – | View 3 publications |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Bacteria, Human |
| Host / Isotype | Rabbit |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E.coli-derived Recombinant Human RANTES (CCL5) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.1–1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | –20°C |
| Shipping Conditions | Ambient |
| RRID | AB_2930485 |
Product Specific Information
AA Sequence of recombinant protein:
SPYSSDTTPC CFAYIARPLP RAHIKEYFYT SGKCSNPAVV FVTRKNRQVC ANPEKKWVRE YINSLEMS
Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human RANTES (CCL5). Anti-Human RANTES (CCL5)-specific antibody was purified by affinity chromatography employing an immobilized Human RANTES (CCL5) matrix.
Sandwich ELISA:
For detection of Human RANTES (CCL5) by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.5–2.0 µg/mL is recommended. When used with PeproTech Biotinylated Anti-Human RANTES (CCL5) (500-P36Bt) as a detection antibody, it enables detection of at least 0.2–0.4 ng/well of Recombinant Human RANTES (CCL5).
Western Blot:
To detect hRANTES by Western blot analysis, use at 0.1–0.2 µg/mL. In conjunction with compatible secondary reagents, the detection limit for Recombinant hRANTES is 1.5–3.0 ng/lane under either reducing or non-reducing conditions.
Target Information
CCL5 (RANTES) is a chemokine gene located on the q-arm of chromosome 17. Chemokines are a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes.
CCL5 functions as a chemoattractant for blood monocytes, memory T helper cells, and eosinophils, and induces histamine release from basophils. It is a major HIV-suppressive factor produced by CD8+ cells and acts as a natural ligand for CCR5, suppressing replication of R5 strains of HIV-1.
Alternative splicing results in multiple transcript variants encoding different isoforms. Diseases associated with CCL5 include Human Immunodeficiency Virus Type 1 and Periapical Granuloma.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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