Thermo Fisher Scientific CCL5 (RANTES) Polyclonal Antibody, PeproTech

Thermo Fisher Scientific CCL5 (RANTES) Polyclonal Antibody, PeproTech

Applications and Tested Dilution

Product Specifications

Product Specific Information

AA Sequence of recombinant protein:
SPYSSDTTPC CFAYIARPLP RAHIKEYFYT SGKCSNPAVV FVTRKNRQVC ANPEKKWVRE YINSLEMS

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human RANTES (CCL5). Anti-Human RANTES (CCL5)-specific antibody was purified by affinity chromatography employing an immobilized Human RANTES (CCL5) matrix.

Sandwich ELISA:
For detection of Human RANTES (CCL5) by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.5–2.0 µg/mL is recommended. When used with PeproTech Biotinylated Anti-Human RANTES (CCL5) (500-P36Bt) as a detection antibody, it enables detection of at least 0.2–0.4 ng/well of Recombinant Human RANTES (CCL5).

Western Blot:
To detect hRANTES by Western blot analysis, use at 0.1–0.2 µg/mL. In conjunction with compatible secondary reagents, the detection limit for Recombinant hRANTES is 1.5–3.0 ng/lane under either reducing or non-reducing conditions.

Target Information

CCL5 (RANTES) is a chemokine gene located on the q-arm of chromosome 17. Chemokines are a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes.
CCL5 functions as a chemoattractant for blood monocytes, memory T helper cells, and eosinophils, and induces histamine release from basophils. It is a major HIV-suppressive factor produced by CD8+ cells and acts as a natural ligand for CCR5, suppressing replication of R5 strains of HIV-1.
Alternative splicing results in multiple transcript variants encoding different isoforms. Diseases associated with CCL5 include Human Immunodeficiency Virus Type 1 and Periapical Granuloma.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.