Thermo Fisher Scientific VISTA Polyclonal Antibody
Human VISTA 단백질을 인식하는 Rabbit Polyclonal Antibody로, WB 및 IHC(P) 실험에 적합합니다. 항원 친화 크로마토그래피로 정제되었으며, PBS와 40% glycerol buffer에 보관됩니다. 암 면역치료 연구 및 T세포 활성 억제 기전 연구에 활용됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.04–0.4 µg/mL | - |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:500 | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human C10orf54. Recombinant protein control fragment (Product # RP-90094) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping conditions | Wet ice |
| RRID | AB_2638669 |
Product Specific Information
Immunogen sequence:
LTFQDLHLHH GGHQAANTSH DLAQRHGLES ASDHHGNFSI TMRNLTLLDS GLYCCLVVEI RHHHSEHRVH GAMELQVQTG KDAPSNCVVY PSSS
Antigen sequence identity:
- Mouse: 60%
- Rat: 59%
Target Information
VISTA (V-domain Ig containing suppressor of T-cell activation), also known as B7-H5 or PD-1H, is encoded by the VSIR gene.
Although VISTA shares sequence homology with PD-L1, it exhibits a distinct expression pattern. In both mouse and human, VISTA is expressed predominantly on hematopoietic cells — highest on myeloid cells and lower on T cells, while typically absent on B cells.
VISTA expressed by antigen-presenting cells can directly suppress proliferation and cytokine production of CD4 and CD8 T cells, making it an important target for cancer immunotherapy.
Reported ligands include CD28H, VSIG-3, and VSIG-8.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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