Thermo Fisher Scientific CD53 Recombinant Mouse Monoclonal Antibody (161-2 (53/2))
인간 CD53을 특이적으로 인식하는 재조합 마우스 단클론 항체. HEK293 세포에서 발현되며 Protein A로 정제됨. ICC/IF, Flow Cytometry, Functional Assay에 사용 가능. 비결합형 액상 형태로 1 mg/mL 농도 제공. 연구용 전용 시약.
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Applications
Immunocytochemistry (ICC/IF)
- Tested Dilution: Assay-dependent
Flow Cytometry (Flow)
- Tested Dilution: Assay-dependent
Functional Assay
- Tested Dilution: Assay-dependent
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a, kappa |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 161-2 (53/2) |
| Immunogen | This antibody was raised by immunizing mice with stimulated human leukocytes. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
Product Specific Information
This antibody recognizes human CD53. It does not react with the baboon and horse antigen. CD53 is a member of the tetraspanin family and is involved in cellular activation as part of a signal transduction complex involving other membrane glycoproteins.
Target Information
CD53 is a tetraspanin family transmembrane glycoprotein expressed in the lymphoid-myeloid lineage. It forms complexes with leukocyte surface proteins such as CD2, CD19, CD21, MHC II, VLA-4, or other tetraspanins (CD37, CD81, CD82), modulating various signaling processes. CD53 contributes to radioresistancy of tumor cells and exhibits anti-apoptotic effects when triggered. In thymus, CD53 is up-regulated during T cell positive selection and strongly expressed upon macrophage exposure to bacterial lipopolysaccharide, while neutrophil stimulation leads to down-regulation of CD53 expression.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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