Thermo Fisher Scientific CD3 Monoclonal Antibody (17A2), PE-Cyanine7, eBioscience
마우스 CD3 복합체를 인식하는 17A2 단클론 항체로, PE-Cyanine7 형광체가 결합되어 있습니다. Flow cytometry에 최적화되어 있으며, 높은 특이성과 안정성을 제공합니다. 4°C에서 암소 보관하며, 광산화에 민감하므로 빛을 피해야 합니다.
- 카탈로그번호
- 25-0032-82
- 판매단위
- pk
카탈로그
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Thermo Fisher Scientific CD3 Monoclonal Antibody (17A2), PE-Cyanine7, eBioscience
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 3 publications |
| Immunohistochemistry (Frozen) (IHC (F)) | - | View 1 publication |
| Immunocytochemistry (ICC/IF) | - | View 2 publications |
| Flow Cytometry (Flow) | 0.5 µg/test | View 34 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host / Isotype | Rat / IgG2b, kappa |
| Recommended Isotype Control | Rat IgG2b kappa Isotype Control (eB149/10H5), PE-Cyanine7, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 17A2 |
| Conjugate | PE-Cyanine7 |
| Excitation / Emission Max | 569 / 780 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2815096 |
Available Formats
- Unconjugated
- Alexa Fluor 488
- Alexa Fluor 532
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 395, 496, 737, 805
- Brilliant Violet 421, 480, 650
- eFluor 450, 506, 660
- FITC
- Functional Grade
- PE, PE-Cyanine5, PE-eFluor 610
- PerCP-eFluor 710
- Super Bright 600, 702, 780
- Request custom conjugation
Product Specific Information
The 17A2 monoclonal antibody reacts with the mouse CD3 complex. CD3 subunits gamma, delta, and epsilon are required for proper assembly, trafficking, and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells. Binding of 17A2 to CD3 initiates intracellular signaling resulting in activation and proliferation.
Applications Reported: Flow cytometric analysis.
Applications Tested: Tested on mouse splenocytes (≤0.5 µg/test). Recommended titration for optimal assay performance.
Light Sensitivity: Protect from light due to photo-induced oxidation.
Fixation: Samples can be stored up to 3 days at 4°C in the dark using IC Fixation Buffer (#00-8222-49) or 1-step Fix/Lyse Solution (#00-5333-57).
Excitation: 488–561 nm; Emission: 775 nm; Laser: Blue, Green, Yellow-Green.
Target Information
The CD3 complex plays a crucial role in transducing antigen-recognition signals into T cells and regulating TCR surface expression. It consists of CD3 gamma, delta, epsilon, and zeta subunits, members of the immunoglobulin superfamily encoded on chromosome 11. CD3 is expressed on 68–82% of peripheral blood lymphocytes and 65–85% of thymocytes, but not on B or NK cells. Defects in CD3 genes are associated with CD3 immunodeficiency.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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