Thermo Fisher Scientific NTH1 Polyclonal Antibody, FITC
FITC로 표지된 NTH1 폴리클로날 항체로, 인간 NTHL1 단백질의 N-말단 영역을 인식합니다. Western blot, ELISA, IP에 적합하며, 형광 분석용으로 사용됩니다. 친화 크로마토그래피로 정제되어 높은 특이성과 재현성을 제공합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| ELISA | 1:10,000 |
| Immunoprecipitation (IP) | 1:50–1:250 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | N-epitope synthetic peptide within amino acid region 1–100 on human NTHL1 protein |
| Conjugate | FITC (Fluorescein) |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 0.5–1.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | Proprietary buffer, pH 7.4–7.8, with 30% glycerol, 0.5% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | –20°C, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Oxygen radicals damage chromosomal DNA, causing cell death and mutations. Although much focus has been placed on repairing 8-oxo-G, many other oxidatively damaged DNA sites are created by free-radical attack.
The human NTH1 repair protein acts on a wide range of these damaged sites. A homologue of E. coli Endonuclease III, NTH is a DNA glycosylase with apurinic/apyrimidinic lyase activity.
The NTH protein has broad substrate specificity, including numerous ring saturation and fragmentation products of pyrimidines. Research indicates NTH plays a crucial role in removing oxidative base lesions in mitochondrial DNA.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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