Thermo Fisher Scientific NTH1 Polyclonal Antibody, Biotin
Human NTH1 단백질을 인식하는 Biotin 표지 Rabbit Polyclonal 항체. Western blot, ELISA, Immunoprecipitation에 사용 가능. 산화적 DNA 손상 복구 연구에 적합. Affinity chromatography로 정제된 고순도 항체.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| ELISA | 1:10,000 |
| Immunoprecipitation (IP) | 1:50–1:250 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | N-epitope synthetic peptide taken within amino acid region 1–100 on human NTHL1 protein |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 0.5–1.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | Proprietary buffer, pH 7.4–7.8, with 30% glycerol, 0.5% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Oxygen radicals damage chromosomal DNA, causing cell death and inducing mutations. Although a major focus of oxidatively damaged DNA has centered on the repair of 8-oxo-G, many other damaged DNA sites are created by free-radical attack on DNA.
The human NTH1 repair protein is one enzyme that acts on a large number of these oxidatively damaged DNA sites. A homologue of E. coli Endonuclease III, NTH is a DNA glycosylase with apurinic/apyrimidinic lyase activity.
The NTH protein has broad substrate specificity, including numerous ring saturation and fragmentation products of pyrimidines. Research indicates that NTH plays a crucial role in the removal of oxidative base lesions in mitochondrial DNA.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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