Thermo Fisher Scientific ERCC3 Polyclonal Antibody
ERCC3 단백질을 인식하는 Rabbit Polyclonal 항체로, Western blot, IHC, IP에 사용 가능. Human에 반응하며 Mouse, Rat 등에도 예측 반응. 항원 친화 크로마토그래피로 정제된 액상 형태로 4°C 보관. 연구용으로만 사용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | Assay-dependent |
| Immunohistochemistry (IHC) | 1:2,000 to 1:10,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:2,000 to 1:10,000 |
| Immunoprecipitation (IP) | 2–5 µg/mg lysate |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 575 and 625 of human excision repair cross-complementing rodent repair deficiency, complementation group 3 (XPB, UniProt ID: P19447-1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | Tris citrate/phosphate, pH 7–8 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- For IHC, epitope retrieval with citrate buffer (pH 6.0) is recommended for FFPE tissue sections
- Based on 100% sequence identity, this antibody is predicted to react with Mouse, Rat, Zebrafish, Bovine, and Orangutan
Target Information
The human XPB DNA helicase is a subunit of the DNA repair/basal transcription factor TFIIH, involved in early steps of the nucleotide excision repair (NER) pathway.
Mutations in the XPB gene can lead to clinical phenotypes such as xeroderma pigmentosum associated with Cockayne’s syndrome (XP/CS) and trichothiodystrophy (TTD).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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