Thermo Fisher Scientific CD68 Monoclonal Antibody (FA-11), Brilliant Ultra Violet 805, eBioscience

Thermo Fisher Scientific CD68 Monoclonal Antibody (FA-11), Brilliant Ultra Violet 805, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 1.0 µg/test

Product Specifications

Additional Formats

• Unconjugated
• Alexa Fluor 488
• APC-eFluor 780
• Brilliant Violet 421
• Brilliant Violet 786
• eFluor 660
• PE
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Request custom conjugation

Product Specific Information

This FA-11 monoclonal antibody reacts with mouse CD68 (macrosialin), a type I integral membrane glycoprotein of the sialomucin family related to LAMP proteins. CD68 is primarily localized in late endosomes and lysosomes, with a smaller fraction at the cell surface. It is a pan-macrophage marker and also expressed at low levels in Langerhans and dendritic cells. CD68 binds oxidized LDL and participates in phagocytosis.

Applications Reported: Intracellular staining followed by flow cytometric analysis.
Applications Tested: Tested on mouse bone marrow cells using Intracellular Fixation & Permeabilization Buffer Set (Product #88-8824-00). Recommended ≤1.0 µg/test. Optimize titration for best performance.

Fluorochrome Information:
Brilliant Ultra Violet™ 805 (BUV805) emits at 797 nm and is for use with 355 nm UV laser-equipped cytometers. Use Brilliant Stain Buffer™ or Super Bright Complete Staining Buffer to minimize non-specific polymer interactions.

Light Sensitivity: Protect from light.
Fixation: Compatible with short-term methanol fixation (<1 hr); store fixed samples at 4°C in the dark for up to 3 days.
Excitation / Emission: 355 nm / 797 nm
Laser: Ultraviolet Laser

BRILLIANT ULTRA VIOLET™ is a trademark of Becton, Dickinson and Company or its affiliates, used under license. Powered by Sirigen.

Target Information

CD68 (Macrosialin) is a 110 kDa integral membrane glycoprotein expressed mainly on lysosomes of monocytes and macrophages, and to a lesser extent in dendritic cells and granulocytes. It plays roles in phagocytosis and cell-pathogen interactions. Expression is observed in tonsil interdigitating reticulum cells, histiocytic lymphoma, AML, and granulocytic sarcoma. Elevated CD68 expression is noted in CD34+ cells in various malignancies.

For Research Use Only. Not for use in diagnostic procedures.

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