Thermo Fisher Scientific CD123 Monoclonal Antibody (6H6), Brilliant Ultra Violet 805, eBioscience
인간 CD123(IL-3 수용체 α체인)을 인식하는 Mouse IgG1 단일클론 항체로, Brilliant Ultra Violet 805 형광체 결합. 유세포 분석용으로 최적화되어 있으며, 고감도 신호 검출과 낮은 비특이 결합을 제공. 연구용 시약으로 4°C 암소 보관.
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Thermo Fisher Scientific CD123 Monoclonal Antibody (6H6), Brilliant Ultra Violet 805, eBioscience™
Applications
- Flow Cytometry (Flow)
Tested Dilution: 5 µL (0.25 µg)/test
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Brilliant Ultra Violet™ 805, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 6H6 |
| Conjugate | Brilliant Ultra Violet™ 805 |
| Excitation / Emission Max | 349 / 804 nm |
| Form | Liquid |
| Concentration | 5 µL/test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2896129 |
Available Formats
- Unconjugated
- Alexa Fluor 532
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 395
- Brilliant UV 615
- Brilliant UV 661
- Brilliant UV 737
- Brilliant Violet 480
- Brilliant Violet 786
- eFluor 450
- FITC
- NovaFluor Blue 510
- PE
- PE-Cyanine5
- PE-Cyanine7
- PE-eFluor 610
- PerCP-Cyanine5.5
- Super Bright 436
- Super Bright 600
- Super Bright 645
- Super Bright 702
- Request custom conjugation available
Product Specific Information
The 6H6 monoclonal antibody reacts with human CD123, the alpha chain of the IL-3 receptor. CD123 is a 60–70 kDa transmembrane protein that binds IL-3 with low affinity alone, and with high affinity when associated with CD131. It is expressed by myeloid precursors, macrophages, dendritic cells, mast cells, basophils, and megakaryocytes.
Applications Reported: Flow cytometric analysis.
Applications Tested: Pre-diluted and tested on normal human peripheral blood cells at 5 µL (0.25 µg) per test.
Brilliant Ultra Violet™ 805 (BUV805) is a tandem dye emitting at 797 nm, optimized for UV (355 nm) laser-equipped cytometers. Ensure instrument compatibility before use.
When using multiple polymer dye-conjugated antibodies, use Super Bright Complete Staining Buffer (Product # SB-4401) or Brilliant Stain Buffer (Product # 00-4409-75) to minimize non-specific interactions.
Light Sensitivity: Protect from light to avoid photo-induced oxidation.
Fixation: Compatible with IC Fixation Buffer (Product # 00-8222) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days at 4°C in the dark.
Methanol Compatibility: Suitable for short-term methanol fixation (<1 hour).
Excitation / Emission / Laser: 355 nm / 797 nm / Ultraviolet Laser.
BRILLIANT ULTRA VIOLET™ is a trademark of Becton, Dickinson and Company or its affiliates, used under license. Powered by Sirigen™.
Target Information
IL-3Ra (Interleukin-3 receptor subunit alpha) belongs to the type I cytokine receptor family and forms a heterodimer with the common beta (CDw131) subunit. It mediates IL-3 signaling and receptor activation. Overexpression of IL-3Ra is reported in AML blasts, making it a potential therapeutic target.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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