Thermo Fisher Scientific CD62L (L-Selectin) Monoclonal Antibody (DREG-56 (DREG56)), Brilliant Ultra Violet 805, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 5 µL (0.125 µg)/test

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Product Specifications

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Available Formats

• Unconjugated
• Alexa Fluor 532
• APC
• APC-eFluor 780
• Brilliant UV 496
• Brilliant UV 615
• Brilliant UV 737
• Brilliant Violet 421
• Brilliant Violet 480
• Brilliant Violet 650
• Brilliant Violet 711
• Brilliant Violet 786
• eFluor 450
• FITC
• Functional Grade
• NovaFluor Blue 510
• NovaFluor Blue 555
• NovaFluor Blue 585
• NovaFluor Blue 610-30S
• NovaFluor Blue 660-40S
• NovaFluor Blue 690
• NovaFluor Blue 760
• NovaFluor Red 710
• NovaFluor Red 725
• NovaFluor Red 755
• NovaFluor Yellow 570
• NovaFluor Yellow 590
• NovaFluor Yellow 660
• PE
• PE-Cyanine5
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Request custom conjugation

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Product Specific Information

The DREG-56 monoclonal antibody reacts with human CD62L, a 76 kDa member of the selectin family. CD62L is expressed by neutrophils, monocytes, and subsets of T, B, and NK cells and binds glycosylated, fucosylated, sulfated sialylated glycoproteins including CD34, glycam-1, and MAdCAM-1. These interactions mediate lymphocyte rolling on activated endothelium and homing to high endothelial venules (HEV) in peripheral lymphoid tissues.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Pre-diluted and tested by flow cytometry on normal human peripheral blood cells
  • Recommended use: 5 µL (0.125 µg)/test in 100 µL final volume
  • Suitable cell number: 10⁵–10⁸ cells/test

Fluorochrome Information:
Brilliant Ultra Violet™ 805 (BUV805) emits at 797 nm and is designed for UV (355 nm) laser cytometers.

Buffer Recommendations:
When using multiple Brilliant Ultra Violet™, Brilliant Violet™, or polymer dye-conjugated antibodies, use:

• Super Bright Complete Staining Buffer (SB-4401) or
• Brilliant Stain Buffer (00-4409-75)

Light Sensitivity:
Protect from light due to photo-induced oxidation sensitivity.

Fixation:
Samples can be stored up to 3 days at 4°C in the dark using:

• IC Fixation Buffer (00-8222)
• 1-step Fix/Lyse Solution (00-5333)

Methanol Compatibility:
Compatible with short-term methanol fixation (≤1 hour).

Excitation/Emission:
355 nm / 797 nm (Ultraviolet Laser)

BRILLIANT ULTRA VIOLET™ is a trademark of Becton, Dickinson and Company, used under license. Powered by Sirigen™.

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Target Information

Human CD62L (L-Selectin) is a 74–95 kDa glycoprotein adhesion receptor composed of:

• C-type lectin binding domain
• EGF-like domain
• Two SCR sequences
• Transmembrane and cytoplasmic regions

It is constitutively expressed on most leukocytes including lymphocytes (except many memory T cells), monocytes, and polymorphonuclear cells.

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For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.

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