Thermo Fisher Scientific HLA-Pan (MHC II) Recombinant Mouse Monoclonal Antibody (rHLA-Pan, 3475)

Applications

Tested Dilution

Publications

Western Blot (WB)

1-2 µg/mL

-

Immunohistochemistry (Paraffin) (IHC (P))

1-2 µg/mL

-

Immunocytochemistry (ICC/IF)

1-2 µg/mL

-

Flow Cytometry (Flow)

1-2 µg/10^6 cells

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1, kappa

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

rHLA-Pan, 3475

Immunogen

Non-T, non-B human acute lymphoblastic leukemia REH6 cells if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:HLA-DR/DP/DQ,uniProtId:P01911-1,ncbiNodeId:9606,antigenRange:1-266,antigenLength:266,antigenImageFileName:MSM2-3475-P1_HLA-DRDPDQ_P01911-1_House_mouse.svg,antigenImageFileNamePDP:MSM2-3475-P1_HLA-DRDPDQ_P01911-1_House_mouse_PDP.jpeg,sortOrder:1},{targetFamily:HLA-DR/DP/DQ,uniProtId:P01920-1,ncbiNodeId:9606,antigenRange:1-261,antigenLength:261,antigenImageFileName:MSM2-3475-P1_HLA-DRDPDQ_P01920-1_House_mouse.svg,antigenImageFileNamePDP:MSM2-3475-P1_HLA-DRDPDQ_P01920-1_House_mouse_PDP.jpeg,sortOrder:2},{targetFamily:HLA-DR/DP/DQ,uniProtId:P04440-1,ncbiNodeId:9606,antigenRange:1-258,antigenLength:258,antigenImageFileName:MSM2-3475-P1_HLA-DRDPDQ_P04440-1_House_mouse.svg,antigenImageFileNamePDP:MSM2-3475-P1_HLA-DRDPDQ_P04440-1_House_mouse_PDP.jpeg,sortOrder:3}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

200 µg/mL

Purification

Protein A/G

Storage buffer

PBS, pH 7.4, with 0.05% BSA

Contains

0.05% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Immunohistochemistry (PFA fixed): incubate antibody for 30 min RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 degrees C followed by cooling at RT for 20 minutes.

Target Information

MHC class II molecules are transmembrane glycoproteins composed of an alpha chain (36 kDa) and a beta chain (27 kDa). They are expressed primarily on antigen presenting cells such as B lymphocytes, monocytes, macrophages, thymic epithelial cells and activated T lymphocytes. Three loci, DR, DQ and DP, encode the major expressed products of the human class II region. The human MHC class II molecules bind intracellularly processed peptides and present them to T-helper cells. They therefore have a critical role in the initiation of the immune response.

HLA and MHC antibodies play a significant role in Immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem Mass Spectrometry.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.