Thermo Fisher Scientific HLA-Pan (MHC II) Recombinant Mouse Monoclonal Antibody (rHLA-Pan, 3475)
HLA-DR/DP/DQ 항원을 인식하는 재조합 마우스 단일클론 항체로, WB, IHC, ICC/IF, Flow Cytometry에 사용 가능. 높은 특이성과 재현성을 제공하며, HEK293 발현 시스템 기반으로 정제된 고순도 항체. 연구용으로만 사용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10^6 cells |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | rHLA-Pan, 3475 |
| Immunogen | Non-T, non-B human acute lymphoblastic leukemia REH6 cells |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Immunohistochemistry (PFA fixed): incubate antibody for 30 min at room temperature.
- Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C, followed by cooling at room temperature for 20 minutes.
Target Information
MHC class II molecules are transmembrane glycoproteins composed of an alpha chain (36 kDa) and a beta chain (27 kDa). They are expressed primarily on antigen-presenting cells such as B lymphocytes, monocytes, macrophages, thymic epithelial cells, and activated T lymphocytes.
Three loci—DR, DQ, and DP—encode the major expressed products of the human class II region.
Human MHC class II molecules bind intracellularly processed peptides and present them to T-helper cells, playing a critical role in initiating immune responses.
HLA and MHC antibodies are essential tools in Immunopeptidomics, aiding in the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem mass spectrometry.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
제품 이미지
(해당 항원 관련 이미지: MSM2-3475-P1_HLA-DRDPDQ_P01911-1_House_mouse.svg, MSM2-3475-P1_HLA-DRDPDQ_P01911-1_House_mouse_PDP.jpeg 등)
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