Thermo Fisher Scientific PHKG2 Monoclonal Antibody (OTI1F9), TrueMAB
PHKG2 단백질을 인식하는 Mouse Monoclonal Antibody (Clone OTI1F9). Western blot과 IHC(P)에서 검증됨. 동결건조 형태로 제공되며, PBS buffer(8% trehalose) 기반. 연구용으로 PHKG2 관련 질환 연구에 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1F9 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 1–216 of human PHKG2 produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
PHKG2 encodes the hepatic/testis isoform of the phosphorylase kinase gamma subunit.
Phosphorylase kinase is a complex enzyme composed of 16 subunits (four each of α, β, γ, and δ).
- The γ subunit contains the catalytic site.
- The α and β subunits have regulatory roles controlled by phosphorylation.
- The δ subunit (calmodulin) mediates calcium dependence.
Mutations in PHKG2 are associated with glycogen storage disease type 9C (autosomal liver glycogenosis).
Alternative splicing results in multiple isoforms of PHKG2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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