Thermo Fisher Scientific Phospho-ERK1/ERK2 (Thr202, Tyr204, Thr185, Tyr187) Recombinant Rabbit Monoclonal Antibody (HL173)
ERK1/ERK2 인산화 상태(Thr202/Tyr204, Thr185/Tyr187)를 검출하는 재조합 토끼 단클론 항체. Western blot 및 IHC(P)에서 사용 가능. Human, Mouse 반응성. Protein A 정제, 1 mg/mL 농도, 보존제 없음.
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Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:3,000 | - |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | HL173 |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187). The exact sequence is proprietary. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2890401 |
Product Specific Information
Positive control:
Neuro2A (50 µM Ethacrynic acid treatment for 2 hr),
A431 (starvation for 16 hr),
A431 (starvation for 16 hr and 200 ng/mL EGF treatment for 0.5 hr).
Target Information
ERK1 and ERK2 are widely expressed and are involved in the regulation of meiosis, mitosis, and postmitotic functions in differentiated cells. Many different stimuli, including growth factors, cytokines, virus infection, ligands for heterotrimeric guanine nucleotide-binding protein (G protein)-coupled receptors, and transforming agents, activate the ERK1 and ERK2 pathways. When growth factors bind to the receptor tyrosine kinase, Ras interacts with Raf, the serine/threonine protein kinase, and activates it. Once activated, Raf phosphorylates serine residues in two further kinases, MEK1/2, which in turn phosphorylate tyrosine/threonine in extracellular-signal regulated kinase (ERK) 1/2. Upon activation, the ERKs either phosphorylate a number of cytoplasmic targets or migrate to the nucleus, where they phosphorylate and activate transcription factors such as c-Fos and Elk-1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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