Thermo Fisher Scientific Phospho-ERK1/ERK2 (Thr202, Tyr204, Thr185, Tyr187) Recombinant Rabbit Monoclonal Antibody (HL173)

Applications

Product Specifications

Product Specific Information

Positive control:
Neuro2A (50 µM Ethacrynic acid treatment for 2 hr),
A431 (starvation for 16 hr),
A431 (starvation for 16 hr and 200 ng/mL EGF treatment for 0.5 hr).

Target Information

ERK1 and ERK2 are widely expressed and are involved in the regulation of meiosis, mitosis, and postmitotic functions in differentiated cells. Many different stimuli, including growth factors, cytokines, virus infection, ligands for heterotrimeric guanine nucleotide-binding protein (G protein)-coupled receptors, and transforming agents, activate the ERK1 and ERK2 pathways. When growth factors bind to the receptor tyrosine kinase, Ras interacts with Raf, the serine/threonine protein kinase, and activates it. Once activated, Raf phosphorylates serine residues in two further kinases, MEK1/2, which in turn phosphorylate tyrosine/threonine in extracellular-signal regulated kinase (ERK) 1/2. Upon activation, the ERKs either phosphorylate a number of cytoplasmic targets or migrate to the nucleus, where they phosphorylate and activate transcription factors such as c-Fos and Elk-1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.