Thermo Fisher Scientific Phospho-p70 S6 Kinase (Thr421, Ser424) Recombinant Rabbit Monoclonal Antibody (HL129)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

-

Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

HL129

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human p70 S6K (phospho Thr421/Ser424). The exact sequence is proprietary. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:p70 S6 Kinase,uniProtId:P23443-1,ncbiNodeId:9606,antigenRange:424,antigenLength:525,antigenImageFileName:MA5-36267_p70_S6_Kinase_P23443-1_Rabbit.svg,antigenImageFileNamePDP:MA5-36267_p70_S6_Kinase_P23443-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

no preservative

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2890402

Product Specific Information

Positive Control: 293T (starvation for 24 hr and incubated in 20% FBS for 0.5 hr).

Target Information

RPS6KB1 (p70S6K) is a serine/threonine kinase with 2 non-identical kinase catalytic domains which phosphorylates several residues of the S6 ribosomal protein. p70 S6 kinase is predominantly localized in the cytoplasm, and is essential in growth factors regulated cell proliferation, pathways involving cell motility, such as metastases, the immune response, and tissue repair. p70 S6 kinase acts downstream of phosphoinositide (PI) 3-kinase, and its main physiological target is the S6 ribosomal protein, which is involved in upregulation of protein synthesis. The kinase activity of p70 S6 kinase leads to an increase in protein synthesis and cell proliferation. Amplification of the region of DNA encoding p70 S6 kinase and overexpression are seen in some breast cancer cell lines.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.