Thermo Fisher Scientific RGMA Monoclonal Antibody (275407)

Applications

Tested Dilution

Publications

Western Blot (WB)

1 µg/mL

-

Product Specifications

Species Reactivity

Chicken, Human, Mouse

Host/Isotype

Rat / IgG2a

Class

Monoclonal

Type

Antibody

Clone

275407

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse RGM-A Cys48-Gly421 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:RGMA,uniProtId:Q6PCX7-1,ncbiNodeId:10090,antigenRange:48-421,antigenLength:454,antigenImageFileName:MA5-23977_RGMA_Q6PCX7-1_Rat.svg,antigenImageFileNamePDP:MA5-23977_RGMA_Q6PCX7-1_Rat_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.5 mg/mL

Purification

Protein A/G

Storage buffer

PBS with 5% trehalose

Contains

No Preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2606167

Product Specific Information

In direct ELISAs and Western blots, 100% cross-reactivity with recombinant human RGM-A and recombinant chicken RGM is observed. In Western blots, approximately 25% cross-reactivity with recombinant mouse RGM-C is observed.

Reconstitute at 0.5 mg/mL in sterile PBS.

Target Information

Member of the repulsive guidance molecule (RGM) family that performs several functions in the developing and adult nervous system. Regulates cephalic neural tube closure, inhibits neurite outgrowth and cortical neuron branching, and the formation of mature synapses. Binding to its receptor NEO1/neogenin induces activation of RHOA-ROCK1/Rho-kinase signaling pathway through UNC5B-ARHGEF12/LARG-PTK2/FAK1 cascade, leading to collapse of the neuronal growth cone and neurite outgrowth inhibition. Furthermore, RGMA binding to NEO1/neogenin leads to HRAS inactivation by influencing HRAS-PTK2/FAK1-AKT1 pathway. It also functions as a bone morphogenetic protein (BMP) coreceptor that may signal through SMAD1, SMAD5, and SMAD8.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.