Thermo Fisher Scientific TPL2 Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:500–1:3,000

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1:100–1:1,000

Product Specifications

Product Specific Information

• Recommended positive controls: 293T, A431, HeLa, HepG2, Raji, mouse liver
• Predicted reactivity: Mouse (80%), Pig (80%)
• Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

MAP3K8 (COT) is a serine/threonine kinase that activates both MAP and JNK kinase pathways and plays a role in the cell cycle.
Mitogen-activated protein kinase (MAPK) signaling cascades include MAPK or ERK, MAPK kinase (MKK or MEK), and MAPK kinase kinase (MAPKKK or MEKK).
MAPKKK/MEKK phosphorylates and activates MAPK kinase/MEK, which in turn activates MAPK.
The kinases of these signaling cascades are highly conserved, and homologs exist in yeast, Drosophila, and mammalian cells.
MEKK8 activates NF-kappa-B1 via proteasome-mediated proteolysis of NF-kappa-B1/p105.
This cytoplasmic protein is expressed in several normal tissues and tumor-derived cell lines.
The 58 kDa form is specifically activated during S and G2/M phases of the cell cycle.
The longer form undergoes phosphorylation mainly on Ser residues, and the shorter form on both Ser and Thr residues.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.